UBE2I antibody (Middle Region)

Catalog No. ABIN2775681

Product Details anti-UBE2I Antibody

Target: UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Dog, Horse, Cow, Rabbit, Guinea Pig, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2I antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: KDWRPAITIK QILLGIQELL NEPNIQDPAQ AEAYTIYCQN RVEYEKRVRA

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against UBE2I. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human UBE2I

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 474 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for UBE2I

Target: UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))

Alternative Name: UBE2I (UBE2I Products)

Synonyms: C358B7.1 antibody, P18 antibody, UBC9 antibody, ubc9 antibody, ube2ia antibody, zUbc9 antibody, 5830467E05Rik antibody, F830028O17Rik antibody, Ubce2i antibody, Ubce9 antibody, UbcE2A antibody, ubce9 antibody, T13J8.70 antibody, T13J8_70 antibody, UBIQUITIN-PROTEIN LIGASE antibody, ubiquitin conjugating enzyme 9 antibody, ubiquitin conjugating enzyme E2 I antibody, ubiquitin-conjugating enzyme E2Ib antibody, ubiquitin-conjugating enzyme E2L antibody, ubiquitin-conjugating enzyme E2I antibody, ubiquitin conjugating enzyme E2I S homeolog antibody, ubiquitin conjugating enzyme 9 antibody, SUMO-conjugating enzyme UBC9 antibody, UBE2I antibody, ube2ib antibody, UBE2L antibody, Ube2i antibody, ube2i.S antibody, UBC9 antibody, ubc-9 antibody, LOC108703134 antibody

Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2I is a member of the E2 ubiquitin-conjugating enzyme family.
Alias Symbols: C358B7.1, P18, UBC9
Protein Interaction Partner: TRIM21, SP100, SUMO2, RANGAP1, PTEN, LMO2, PIAS2, TRIM23, PARP1, PIAS4, UBXN1, USP25, SETBP1, SIRT1, PUF60, HMGA1, GOLGA2, FHL3, ELK1, DES, DAXX, CREBBP, CLK2, RCBTB2, CEBPD, CBS, CAPN2, PPARGC1A, RFPL3, TRIM38, IKZF1, RNF40, XBP1, KRT19, HNRNPC, ZBTB8A,
Protein Size: 474

Molecular Weight: 52 kDa

Gene ID: 7329

NCBI Accession: NM_018045, NP_003336

UniProt: P63279

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Ubiquitin Proteasome Pathway