Parkin antibody (C-Term)

Catalog No. ABIN2775689

This Rabbit Polyclonal antibody specifically detects Parkin in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Pig and Dog.

Quick Overview for Parkin antibody (C-Term) (ABIN2775689)

Target: Parkin (PARK2) (Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2))

Reactivity: Human, Mouse, Rat, Cow, Pig, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Parkin antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Parkin Antibody

Binding Specificity: C-Term

Sequence: YTRYQQYGAE ECVLQMGGVL CPRPGCGAGL LPEQGQRKVT CEGGNGLGCG

Predicted Reactivity: Cow: 92%, Dog: 86%, Human: 100%, Mouse: 86%, Pig: 93%, Rat: 86%

Characteristics: This is a rabbit polyclonal antibody against Park2. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide corresponding to a region of Mouse

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 464 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for Parkin

Target: Parkin (PARK2) (Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2))

Alternative Name: Park2

Background: Park2 functions within a multiprotein E3 ubiquitin ligase complex, catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. These substrates include SYT11, CCNE1, GPR37, STUB1, a 22 kDa O-linked glycosylated isoform of SNCAIP, SEPT5 and AIMP2. Park2 may play a more general role in the ubiquitin proteasomal pathway by participating in the removal and/or detoxification of abnormally folded or damaged protein. Park2 limits the production of reactive oxygen species (ROS). Loss of this ubiquitin ligase activity appears to be the mechanism underlying pathogenesis of AR-JP. Park2 may protect neurons against alpha synuclein toxicity, proteasomal dysfunction, GPR37 accumulation, and kainate-induced excitotoxicity. Park2 may play a role in controlling neurotransmitter trafficking at the presynaptic terminal and in calcium-dependent exocytosis. Park2 regulates cyclin E during neuronal apoptosis. Park2 may represent a tumor suppressor gene. Park2 promotes the autophagic degradation of dysfunctional depolarized mitochondria.
Alias Symbols: MGC130518, PRKN
Protein Interaction Partner: Becn1, Actb, Hdac6, TARDBP, Bax, Acta1, Ubc, Prps1, SNCA, Sh3gl2, Eps15, Synj1, Sh3gl3, Sh3gl1, Ambra1, Pick1, Cask, Abl1, Bcl2,
Protein Size: 464

Molecular Weight: 51 kDa

Gene ID: 50873

NCBI Accession: NM_016694, NP_057903

UniProt: Q9WVS6

Pathways: Autophagy, Ubiquitin Proteasome Pathway