UBE4A antibody (Middle Region)

Catalog No. ABIN2775692

This Rabbit Polyclonal antibody specifically detects UBE4A in WB. It exhibits reactivity toward Human, Mouse, Rat, Dog, Guinea Pig, Horse, Cow, Rabbit and Zebrafish (Danio rerio).

Quick Overview for UBE4A antibody (Middle Region) (ABIN2775692)

Target: UBE4A (Ubiquitination Factor E4A (UBE4A))

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Horse, Cow, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE4A antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-UBE4A Antibody

Binding Specificity: Middle Region

Purpose: UBE4A Antibody - middle region

Sequence: QYAPQLAEAL IKVFVDIEFT GDPHQFEQKF NYRRPMYPIL RYMWGTDTYR

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against UBE4A. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human UBE4A

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP43044-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for UBE4A

Target: UBE4A (Ubiquitination Factor E4A (UBE4A))

Alternative Name: UBE4A

Background:

Background Information: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE4A is an additional conjugation factor, E4, which is involved in multiubiquitin chain assembly.The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes an additional conjugation factor, E4, which is involved in multiubiquitin chain assembly.

Gene Name: Ubiquitination factor E4A

Alternative Symbols: E4, UFD2, UBOX2

Protein Name: Ubiquitin conjugation factor E4 A

Molecular Weight: 123kDa

Gene ID: 9354

NCBI Accession: NP_004779

UniProt: B0YJB6