CCT4 antibody (C-Term)

Catalog No. ABIN2775694

The Rabbit Polyclonal anti-CCT4 antibody has been validated for WB and IHC. It is suitable to detect CCT4 in samples from Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse, Rabbit, Saccharomyces cerevisiae and Zebrafish (Danio rerio). There are 2+ publications available.

Quick Overview for CCT4 antibody (C-Term) (ABIN2775694)

Target: CCT4 (Chaperonin Containing TCP1, Subunit 4 (Delta) (CCT4))

Reactivity: Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse, Rabbit, Saccharomyces cerevisiae, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCT4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-CCT4 Antibody

Binding Specificity: C-Term

Sequence: AFADAMEVIP STLAENAGLN PISTVTELRN RHAQGEKTAG INVRKGGISN

Predicted Reactivity: Cow: 93%, Dog: 93%, Goat: 79%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 93%, Rabbit: 93%, Rat: 100%, Yeast: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against CCT4. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human CCT4

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 539 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-CCT4 antibody (ABIN2775694)

Tarkar, Loges, Slagle, Francis, Dougherty, Tamayo, Shook, Cantino, Schwartz, Jahnke, Olbrich, Werner, Raidt, Pennekamp, Abouhamed, Hjeij, Köhler, Griese, Li, Lemke, Klena, Liu, Gabriel, Tobita et al.: "DYX1C1 is required for axonemal dynein assembly and ciliary motility. ..." in: Nature genetics, Vol. 45, Issue 9, pp. 995-1003, (2013) (PubMed).

Machida, Masutani, Kobayashi, Mikami, Nishino, Miyazawa, Imataka: "Reconstitution of the human chaperonin CCT by co-expression of the eight distinct subunits in mammalian cells." in: Protein expression and purification, Vol. 82, Issue 1, pp. 61-9, (2012) (PubMed).

Target Details for CCT4

Target: CCT4 (Chaperonin Containing TCP1, Subunit 4 (Delta) (CCT4))

Alternative Name: CCT4

Background: The CCT (chaperonin containing TCP-1) complex functions as a molecular chaperone in the eukaryotic cytosol. CCT4 interacts with human cyclin E which has been implicated in positive control of the G1/S phase transition.
Alias Symbols: SRB, Cctd, CCT-DELTA
Protein Interaction Partner: HUWE1, TP53, FUS, CDC20, NEDD1, TUBG1, LGALS3BP, UBC, STAU1, MDM2, RFWD2, VRK2, PRPF40A, CCT7, USP9X, VARS, CCT3, CCT6A, FBXO6, UBD, FBXW4, CDK9, CDK5, CDK2, CD81, IGSF8, HDAC11, HDAC3, RPS6KA1, ILK, ICAM1, PAN2, VCAM1, PPP5C, PEX14, NOS2, US3, FAM86B2, C
Protein Size: 539

Molecular Weight: 58 kDa

Gene ID: 10575

NCBI Accession: NM_006430, NP_006421

UniProt: Q53QP9

Pathways: Embryonic Body Morphogenesis