PDLIM5 antibody (Middle Region)

Catalog No. ABIN2775697

The Rabbit Polyclonal anti-PDLIM5 antibody is suitable to detect PDLIM5 in samples from Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Guinea Pig and Zebrafish (Danio rerio). It has been validated for WB and IHC.

Quick Overview for PDLIM5 antibody (Middle Region) (ABIN2775697)

Target: PDLIM5 (PDZ and LIM Domain 5 (PDLIM5))

Reactivity: Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Guinea Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDLIM5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-PDLIM5 Antibody

Binding Specificity: Middle Region

Purpose: PDLIM5 Antibody - middle region

Sequence: VKIPPKRPPR KHIVERYTEF YHVPTHSDAS KKRLIEDTED WRPRTGTTQS

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 93%, Rabbit: 93%, Rat: 93%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against PDLIM5. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human PDLIM5

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP34274-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for PDLIM5

Target: PDLIM5 (PDZ and LIM Domain 5 (PDLIM5))

Alternative Name: PDLIM5

Background:

Background Information: PDLIM5 is a LIM domain protein. LIM domains are cysteine-rich double zinc fingers composed of 50 to 60 amino acids that are involved in protein-protein interactions. LIM domain-containing proteins are scaffolds for the formation of multiprotein complexes. The proteins are involved in cytoskeleton organization, cell lineage specification, organ development, and oncogenesis. PDLIM5 is also a member of the Enigma class of proteins, a family of proteins that possess a 100-amino acid PDZ domain in the N terminus and 1 to 3 LIM domains in the C terminus. Multiple transcript variants encoding different isoforms have been found for this gene, although not all of them have been fully characterized.The protein encoded by this gene is a LIM domain protein. LIM domains are cysteine-rich double zinc fingers composed of 50 to 60 amino acids that are involved in protein-protein interactions. LIM domain-containing proteins are scaffolds for the formation of multiprotein complexes. The proteins are involved in cytoskeleton organization, cell lineage specification, organ development, and oncogenesis. The encoded protein is also a member of the Enigma class of proteins, a family of proteins that possess a 100-amino acid PDZ domain in the N terminus and 1 to 3 LIM domains in the C terminus. Multiple transcript variants encoding different isoforms have been found for this gene, although not all of them have been fully characterized.

Gene Name: PDZ and LIM domain 5

Alternative Symbols: L9, ENH, LIM, ENH1

Protein Name: PDZ and LIM domain protein 5

Molecular Weight: 24kDa

Gene ID: 10611

NCBI Accession: NP_001011516

UniProt: Q8WVK0