DPF3 antibody (Middle Region)

Catalog No. ABIN2775708

Cited in 2+ publications. The Rabbit Polyclonal anti-DPF3 antibody (ABIN2775708) specifically detects DPF3 in WB. The antibody is reactive with Human, Mouse, Rat, Guinea Pig, Horse, Rabbit, Cow and Dog samples.

Quick Overview for DPF3 antibody (Middle Region) (ABIN2775708)

Target: DPF3 (D4, Zinc and Double PHD Fingers, Family 3 (DPF3))

Reactivity: Human, Mouse, Rat, Guinea Pig, Horse, Rabbit, Cow, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DPF3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-DPF3 Antibody

Binding Specificity: Middle Region

Purpose: DPF3 Antibody - middle region

Sequence: KDGFTSESTT LEALLRGEGV EKKVDAREEE SIQEIQRVLE NDENVEEGNE

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against DPF3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human DPF3

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP34293-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-DPF3 antibody (ABIN2775708)

Theodorou, Speletas, Mamara, Papachristopoulou, Lazou, Scorilas, Katsantoni: "Identification of a STAT5 target gene, Dpf3, provides novel insights in chronic lymphocytic leukemia." in: PLoS ONE, Vol. 8, Issue 10, pp. e76155, (2013) (PubMed).

Hoyal, Kammerer, Roth, Reneland, Marnellos, Kiechle, Schwarz-Boeger, Griffiths, Ebner, Rehbock, Nelson, Braun: "Genetic polymorphisms in DPF3 associated with risk of breast cancer and lymph node metastases." in: Journal of carcinogenesis, Vol. 4, pp. 13, (2005) (PubMed).

Target Details for DPF3

Target: DPF3 (D4, Zinc and Double PHD Fingers, Family 3 (DPF3))

Alternative Name: DPF3

Background:

Background Information: DPF3 is a muscle-specific component of the BAF complex, a multiprotein complex involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). DPF3 specifically binds acetylated lysines on histone 3 and 4 (H3K14ac, H3K9ac, H4K5ac, H4K8ac, H4K12ac, H4K16ac). In the complex, it acts as a tissue-specific anchor between histone acetylations and methylations and chromatin remodeling. It thereby probably plays an essential role in heart and skeletal muscle development.

Gene Name: D4, zinc and double PHD fingers, family 3

Alternative Symbols: CERD4, BAF45C

Protein Name: Zinc finger protein DPF3

Molecular Weight: 40kDa

Gene ID: 8110

NCBI Accession: NP_036206

UniProt: Q92784