ENO1 antibody (C-Term)

Catalog No. ABIN2775757

This Rabbit Polyclonal antibody specifically detects ENO1 in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Guinea Pig, Rabbit, Horse, Dog, Sheep and Saccharomyces cerevisiae.It has been mentioned in 1 publication

Quick Overview for ENO1 antibody (C-Term) (ABIN2775757)

Target: ENO1 (Enolase 1 (ENO1))

Reactivity: Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Guinea Pig, Rabbit, Horse, Dog, Sheep, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ENO1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-ENO1 Antibody

Binding Specificity: C-Term

Purpose: ENO1 Antibody - C-terminal region

Sequence: VVGLCTGQIK TGAPCRSERL AKYNQLLRIE EELGSKAKFA GRNFRNPLAK

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 93%, Rabbit: 100%, Rat: 100%, Sheep: 93%, Yeast: 79%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against ENO1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human ENO1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8099656

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-ENO1 antibody (ABIN2775757)

Rambaruth, Greenwell, Dwek: "The lectin Helix pomatia agglutinin recognizes O-GlcNAc containing glycoproteins in human breast cancer." in: Glycobiology, Vol. 22, Issue 6, pp. 839-48, (2012) (PubMed).

Target Details for ENO1

Target: ENO1 (Enolase 1 (ENO1))

Alternative Name: ENO1

Background:

Background Information: ENO1 is one of three enolase isoenzymes found in mammals, it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome.

Gene Name: Enolase 1, (alpha)

Alternative Symbols: NNE, PPH, MPB1, ENO1L1, HEL-S-17

Protein Name: Alpha-enolase

Molecular Weight: 47kDa

Gene ID: 2023

NCBI Accession: NP_001419

UniProt: Q53HR3