MIER1 antibody (Middle Region)

Catalog No. ABIN2775813

Product Details anti-MIER1 Antibody

Target: MIER1 (Mesoderm Induction Early Response 1 (MIER1))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Dog, Rabbit, Cow, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MIER1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: RRTGDEKGVE AIPEGSHIKD NEQALYELVK CNFDTEEALR RLRF

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against MIER1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human MIER1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 536 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for MIER1

Target: MIER1 (Mesoderm Induction Early Response 1 (MIER1))

Alternative Name: MIER1 (MIER1 Products)

Synonyms: 4933425I22Rik antibody, 5830411K19Rik antibody, mKIAA1610 antibody, mier1 antibody, wu:fb30a02 antibody, zgc:56179 antibody, mi-er1 antibody, ER1 antibody, MI-ER1 antibody, RGD1562337 antibody, MEIR1 treanscription regulator antibody, MIER1 transcriptional regulator antibody, mesoderm induction early response 1a, transcriptional regulator antibody, mesoderm induction early response 1, transcriptional regulator L homeolog antibody, Mier1 antibody, MIER1 antibody, mier1a antibody, mier1.L antibody

Background: The association of hMI-ER1 with Sp1 represents a novel mechanism for the negative regulation of Sp1 target promoters. Results demonstrate that alternate use of a facultative intron regulates the subcellular localization of hMI-ER1 proteins and this may have important implications for hMI-ER1 function in the regulation of transcription. Repressor activity is due to interaction and recruitment of a trichostatin A-sensitive histone deacetylase 1 (HDAC1).
Alias Symbols: ER1, MI-ER1, hMI-ER1, RP5-944N15.1
Protein Interaction Partner: TMEM171, SOX2, HDAC2, HDAC1, EHMT2, CDYL, TUBB1, APP, ELAVL1, UBC, CREBBP, SP1,
Protein Size: 536

Molecular Weight: 49 kDa

Gene ID: 57708

NCBI Accession: NM_020948, NP_065999

UniProt: Q8NES5

Pathways: Chromatin Binding