TFB2M antibody (N-Term)

Catalog No. ABIN2775816

The Rabbit Polyclonal anti-TFB2M antibody is suitable to detect TFB2M in samples from Human, Rat, Horse and Rabbit. It has been validated for WB and IHC.

Quick Overview for TFB2M antibody (N-Term) (ABIN2775816)

Target: TFB2M (Transcription Factor B2, Mitochondrial (TFB2M))

Reactivity: Human, Rat, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TFB2M antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-TFB2M Antibody

Binding Specificity: N-Term

Purpose: TFB2M Antibody - N-terminal region

Sequence: ECNPGPGILT QALLEAGAKV VALESDKTFI PHLESLGKNL DGKLRVIHCD

Predicted Reactivity: Horse: 100%, Human: 100%, Rabbit: 83%, Rat: 85%

Characteristics: This is a rabbit polyclonal antibody against TFB2M. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human TFB2M

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP34471-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for TFB2M

Target: TFB2M (Transcription Factor B2, Mitochondrial (TFB2M))

Alternative Name: TFB2M

Background:

Background Information: TFB2M is a S-adenosyl-L-methionine-dependent methyltransferase which specifically dimethylates mitochondrial 12S rRNA at the conserved stem loop. It is also required for basal transcription of mitochondrial DNA, probably via its interaction with POLRMT and TFAM. It stimulates transcription independently of the methyltransferase activity. Compared to TFB1M, it activates transcription of mitochondrial DNA more efficiently, while it has less methyltransferase activity.

Gene Name: Transcription factor B2, mitochondrial

Alternative Symbols: Hkp1, mtTFB2

Protein Name: Dimethyladenosine transferase 2, mitochondrial

Molecular Weight: 45kDa

Gene ID: 64216

NCBI Accession: NP_071761

UniProt: Q9H5Q4