LIN28A antibody (N-Term)

Catalog No. ABIN2775818

The Rabbit Polyclonal anti-LIN28A antibody is suitable to detect LIN28A in samples from Human, Mouse, Rat, Cow, Dog, Pig, Sheep, Goat, Guinea Pig, Rabbit and Saccharomyces cerevisiae. It has been validated for WB and IHC.

Quick Overview for LIN28A antibody (N-Term) (ABIN2775818)

Target: LIN28A (Lin-28 Homolog A (C. Elegans) (LIN28A))

Reactivity: Human, Mouse, Rat, Cow, Dog, Pig, Sheep, Goat, Guinea Pig, Rabbit, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIN28A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-LIN28A Antibody

Binding Specificity: N-Term

Purpose: LIN28 Antibody - N-terminal region

Sequence: GSVSNQQFAG GCAKAAEEAP EEAPEDAARA ADEPQLLHGA GICKWFNVRM

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 91%, Guinea Pig: 86%, Human: 100%, Mouse: 90%, Pig: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%, Yeast: 77%

Characteristics: This is a rabbit polyclonal antibody against LIN28. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human LIN28

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP34477-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for LIN28A

Target: LIN28A (Lin-28 Homolog A (C. Elegans) (LIN28A))

Alternative Name: LIN28

Background:

Background Information: LIN28 acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. The association of LIN28 with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. IT binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. LIN28 is Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression.

Gene Name: Lin-28 homolog A (C. elegans)

Alternative Symbols: CSDD1, LIN28, LIN-28, ZCCHC1, lin-28A

Protein Name: Protein lin-28 homolog A

Molecular Weight: 23kDa

Gene ID: 79727

NCBI Accession: NP_078950

UniProt: Q9H9Z2

Pathways: Stem Cell Maintenance