PHD1 antibody (N-Term)

Catalog No. ABIN2775821

The Rabbit Polyclonal anti-PHD1 antibody has been validated for WB. It is suitable to detect PHD1 in samples from Human, Mouse, Rat, Dog, Cow, Guinea Pig, Rabbit and Pig.

Quick Overview for PHD1 antibody (N-Term) (ABIN2775821)

Target: PHD1 (EGLN2) (Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Rabbit, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PHD1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PHD1 Antibody

Binding Specificity: N-Term

Sequence: SAGSGTPRAT ATSTTASPLR DGFGGQDGGE LRPLQSEGAA ALVTKGCQRL

Predicted Reactivity: Cow: 79%, Dog: 93%, Guinea Pig: 86%, Human: 100%, Mouse: 93%, Pig: 93%, Rabbit: 79%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against EGLN2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human EGLN2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 407 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for PHD1

Target: PHD1 (EGLN2) (Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2))

Alternative Name: EGLN2

Background: The hypoxia inducible factor (HIF) is a transcriptional complex which is involved in oxygen homeostasis. At normal oxygen levels, the alpha subunit of HIF is targeted for degration by prolyl hydroxylation. EGLN2 encodes an enzyme responsible for this posttranslational modification. Alternative splicing of EGLN2 results in three transcript variants encoding different isoforms.
Alias Symbols: EIT6, PHD1, HPH-1, HPH-3, HIFPH1, HIF-PH1
Protein Interaction Partner: SRPK2, CEP192, IKBKG, FAM46A, EPAS1, A2M, MAGEA11, SIAH2, LPP, WTIP, AJUBA, LIMD1, TRIP6, SPRY2, PRPF19, EGLN3, EGLN2, HIF1A, HIF3A, MOB4, POLR2A,
Protein Size: 407

Molecular Weight: 44 kDa

Gene ID: 112398

NCBI Accession: NM_053046, NP_444274

UniProt: Q96KS0

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Cell RedoxHomeostasis