TRIM50 antibody (Middle Region)

Catalog No. ABIN2775829

The Rabbit Polyclonal anti-TRIM50 antibody (ABIN2775829) specifically detects TRIM50 in WB. The antibody is reactive with Human, Cow, Dog, Rat, Horse, Rabbit, Guinea Pig, Mouse and Pig samples.

Quick Overview for TRIM50 antibody (Middle Region) (ABIN2775829)

Target: TRIM50 (Tripartite Motif Containing 50 (TRIM50))

Reactivity: Human, Cow, Dog, Rat, Horse, Rabbit, Guinea Pig, Mouse, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRIM50 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-TRIM50 Antibody

Binding Specificity: Middle Region

Purpose: TRIM50 Antibody - middle region

Sequence: DWRLGVIKGT ASRKGKLNRS PEHGVWLIGL KEGRVYEAFA CPRVPLPVAG

Predicted Reactivity: Cow: 86%, Dog: 86%, Guinea Pig: 86%, Horse: 86%, Human: 100%, Mouse: 93%, Pig: 93%, Rabbit: 79%, Rat: 86%

Characteristics: This is a rabbit polyclonal antibody against TRIM50. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human TRIM50

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP34494-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for TRIM50

Target: TRIM50 (Tripartite Motif Containing 50 (TRIM50))

Alternative Name: TRIM50

Background:

Background Information: TRIM50 belongs to the TRIM/RBCC family. It contains 1 B box-type zinc finger, 1 B30.2/SPRY domain and 1 RING-type zinc finger. TRIM50 is an E3 ubiquitin-protein ligase.

Gene Name: Tripartite motif containing 50

Alternative Symbols: TRIM50A

Protein Name: E3 ubiquitin-protein ligase TRIM50

Molecular Weight: 55kDa

Gene ID: 135892

NCBI Accession: NP_835226

UniProt: Q86XT4