KLHL12 antibody (N-Term)

Catalog No. ABIN2775905

This Rabbit Polyclonal antibody specifically detects KLHL12 in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Horse, Rabbit, Zebrafish (Danio rerio) and Guinea Pig.

Quick Overview for KLHL12 antibody (N-Term) (ABIN2775905)

Target: KLHL12 (Kelch-Like 12 (Drosophila) (KLHL12))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Rabbit, Zebrafish (Danio rerio), Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KLHL12 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-KLHL12 Antibody

Binding Specificity: N-Term

Purpose: KLHL12 Antibody - N-terminal region

Sequence: EKGKPYVDIQ GLTASTMEIL LDFVYTETVH VTVENVQELL PAACLLQLKG

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against KLHL12. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N-terminal region of Human KLHL12

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP34604-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for KLHL12

Target: KLHL12 (Kelch-Like 12 (Drosophila) (KLHL12))

Alternative Name: KLHL12

Background:

Background Information: KLHL12 is a substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a negative regulator of Wnt signaling pathway and ER-Golgi transport. The BCR(KLHL12) complex is involved in ER-Golgi transport by regulating the size of COPII coats, thereby playing a key role in collagen export, which is required for embryonic stem (ES) cells division: BCR(KLHL12) acts by mediating monoubiquitination of SEC31 (SEC31A or SEC31B). As part of the BCR(KLHL12) complex, also acts as a negative regulator of the Wnt signaling pathway by mediating ubiquitination and subsequent proteolysis of DVL3. The BCR(KLHL12) complex also mediates polyubiquitination of DRD4, without leading to degradation of DRD4.

Gene Name: kelch-like 12 (Drosophila)

Alternative Symbols: DKIR, C3IP1

Protein Name: Kelch-like protein 12

Molecular Weight: 63kDa

Gene ID: 59349

NCBI Accession: NP_067646

UniProt: Q53G59