KEAP1 antibody (C-Term)

Catalog No. ABIN309756

This anti-KEAP1 antibody is a Rabbit Polyclonal antibody detecting KEAP1 in WB and IHC. Suitable for Human, Mouse, Rat, Rabbit, Cow, Dog, Horse, Guinea Pig and Zebrafish (Danio rerio). This Primary Antibody has been cited in 1 publication.

Quick Overview for KEAP1 antibody (C-Term) (ABIN309756)

Target: KEAP1 (Kelch-Like ECH-Associated Protein 1 (KEAP1))

Reactivity: Human, Mouse, Rat, Rabbit, Cow, Dog, Horse, Guinea Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KEAP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-KEAP1 Antibody

Binding Specificity: C-Term

Sequence: TWTFVAPMKH RRSALGITVH QGRIYVLGGY DGHTFLDSVE CYDPDTDTWS

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against KEAP1. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human KEAP1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 624 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-KEAP1 antibody (ABIN309756)

Konishi, Baumgarten, Ishida, Saitoh, Anker, Springer: "Protein levels in Keap1-Nrf2 system in human failing heart." in: International journal of cardiology, Vol. 225, pp. 62-64, (2016) (PubMed).

Target Details for KEAP1

Target: KEAP1 (Kelch-Like ECH-Associated Protein 1 (KEAP1))

Alternative Name: KEAP1

Background: KEAP1 contains KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase.This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene.
Alias Symbols: INrf2, KIAA0132, KLHL19, MGC10630, MGC1114, MGC20887, MGC4407, MGC9454
Protein Interaction Partner: OXR1, ZSCAN32, LSM3, KLHL3, FBXW11, MAPKBP1, NUDT4, DPP3, RBX1, ATG5, SQSTM1, PTMA, PRKAG1, NFE2L2, MAD2L1, GYPA, ETF1, BCL2, CUL3, RECK, UBC, PGAM5, FAM117B, WDR83, CCNB1IP1, HSP90AA1, XPO1, MCMBP, KEAP1, SLK, MCM3, IKBKB, AMER1, GPRASP2, PRKCE, NFE2L1,
Protein Size: 624

Molecular Weight: 70 kDa

Gene ID: 9817

NCBI Accession: NM_203500, NP_987096

UniProt: Q14145

Pathways: Maintenance of Protein Location