KEAP1 antibody (C-Term)

Catalog No. ABIN2775979

Cited in 1 publication. The Rabbit Polyclonal anti-KEAP1 antibody (ABIN2775979) specifically detects KEAP1 in WB and IHC. The antibody is reactive with Human, Mouse, Rat, Cow, Dog, Horse, Guinea Pig, Rabbit and Zebrafish (Danio rerio) samples.

Quick Overview for KEAP1 antibody (C-Term) (ABIN2775979)

Target: KEAP1 (Kelch-Like ECH-Associated Protein 1 (KEAP1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Guinea Pig, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KEAP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-KEAP1 Antibody

Binding Specificity: C-Term

Purpose: KEAP1 Antibody - C-terminal region

Sequence: TWTFVAPMKH RRSALGITVH QGRIYVLGGY DGHTFLDSVE CYDPDTDTWS

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against KEAP1. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human KEAP1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8103731

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-KEAP1 antibody (ABIN2775979)

Konishi, Baumgarten, Ishida, Saitoh, Anker, Springer: "Protein levels in Keap1-Nrf2 system in human failing heart." in: International journal of cardiology, Vol. 225, pp. 62-64, (2016) (PubMed).

Target Details for KEAP1

Target: KEAP1 (Kelch-Like ECH-Associated Protein 1 (KEAP1))

Alternative Name: KEAP1

Background:

Background Information: KEAP1 contains KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase.This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene.

Gene Name: Kelch-like ECH-associated protein 1

Alternative Symbols: INrf2, KLHL19

Protein Name: Kelch-like ECH-associated protein 1

Molecular Weight: 70kDa

Gene ID: 9817

NCBI Accession: NP_987096

UniProt: Q14145

Pathways: Maintenance of Protein Location