CHRNA3 antibody (N-Term)

Catalog No. ABIN2776100

The Rabbit Polyclonal anti-CHRNA3 antibody is suitable to detect CHRNA3 in samples from Human, Rat, Mouse, Cow, Dog, Rabbit, Guinea Pig, Horse and Zebrafish (Danio rerio). It has been validated for WB.

Quick Overview for CHRNA3 antibody (N-Term) (ABIN2776100)

Target: CHRNA3 (Cholinergic Receptor, Nicotinic, alpha 3 (Neuronal) (CHRNA3))

Reactivity: Human, Rat, Mouse, Cow, Dog, Rabbit, Guinea Pig, Horse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHRNA3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CHRNA3 Antibody

Binding Specificity: N-Term

Purpose: CHRNA3 Antibody - N-terminal region

Sequence: EHRLFERLFE DYNEIIRPVA NVSDPVIIHF EVSMSQLVKV DEVNQIMETN

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 75%

Characteristics: This is a rabbit polyclonal antibody against CHRNA3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human CHRNA3

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP34964-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for CHRNA3

Target: CHRNA3 (Cholinergic Receptor, Nicotinic, alpha 3 (Neuronal) (CHRNA3))

Alternative Name: CHRNA3

Background:

Targeted Subunit: alpha-3

Background Information: The CHRNA3 subunit is expressed in the soma of the majority of pyramidal cells, with the most alpha 3 immunoreactivity observed in CA2-4 and entorhinal cortex and relatively less in CA1 and subicular pyramidal cell soma.

Gene Name: Cholinergic receptor, nicotinic, alpha 3 (neuronal)

Alternative Symbols: LNCR2, PAOD2, BAIPRCK, NACHRA3

Protein Name: Neuronal acetylcholine receptor subunit alpha-3

Molecular Weight: 57kDa

Gene ID: 1136

NCBI Accession: NP_000734

UniProt: P32297

Pathways: Synaptic Membrane