SMPD1 antibody (Middle Region)

Catalog No. ABIN2776371

The Rabbit Polyclonal anti-SMPD1 antibody is suitable to detect SMPD1 in samples from Human, Mouse, Rat, Cow, Sheep, Dog, Horse, Rabbit, Guinea Pig and Pig. It has been validated for WB.

Quick Overview for SMPD1 antibody (Middle Region) (ABIN2776371)

Target: SMPD1 (Sphingomyelin phosphodiesterase 1, Acid Lysosomal (SMPD1))

Reactivity: Human, Mouse, Rat, Cow, Sheep, Dog, Horse, Rabbit, Guinea Pig, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMPD1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SMPD1 Antibody

Binding Specificity: Middle Region

Purpose: SMPD1 Antibody - middle region

Sequence: INSTDPAGQL QWLVGELQAA EDRGDKVHII GHIPPGHCLK SWSWNYYRIV

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 86%, Pig: 100%, Rabbit: 100%, Rat: 86%, Sheep: 100%

Characteristics: This is a rabbit polyclonal antibody against SMPD1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human SMPD1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP35630-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for SMPD1

Target: SMPD1 (Sphingomyelin phosphodiesterase 1, Acid Lysosomal (SMPD1))

Alternative Name: SMPD1

Background:

Background Information: SMPD1 is a lysosomal acid sphingomyelinase that converts sphingomyelin to ceramide. The encoded protein also has phospholipase C activity. Defects in this gene are a cause of Niemann-Pick disease type A (NPA) and Niemann-Pick disease type B (NPB).

Gene Name: Sphingomyelin phosphodiesterase 1, acid lysosomal

Alternative Symbols: ASM, NPD, ASMASE

Protein Name: Sphingomyelin phosphodiesterase

Molecular Weight: 65kDa

Gene ID: 6609

NCBI Accession: NP_000534

UniProt: P17405