PITX2 antibody (N-Term)

Catalog No. ABIN2776373

There is 1 publication for this product available. The Rabbit Polyclonal anti-PITX2 antibody is suitable to detect PITX2 in samples from Human, Rat, Mouse, Pig, Rabbit, Cow and Dog. It has been validated for WB and IHC.

Quick Overview for PITX2 antibody (N-Term) (ABIN2776373)

Target: PITX2 (Paired-Like Homeodomain 2 (PITX2))

Reactivity: Human, Rat, Mouse, Pig, Rabbit, Cow, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PITX2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-PITX2 Antibody

Binding Specificity: N-Term

Purpose: PITX2 Antibody - N-terminal region

Sequence: SAVSSSSCHH PQPLAMASVL APGQPRSLDS SKHRLEVHTI SDTSSPEAAE

Predicted Reactivity: Cow: 100%, Dog: 93%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 93%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against PITX2. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human PITX2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP35634-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-PITX2 antibody (ABIN2776373)

Hatou, Yoshida, Higa, Miyashita, Inagaki, Okano, Tsubota, Shimmura: "Functional corneal endothelium derived from corneal stroma stem cells of neural crest origin by retinoic acid and Wnt/?-catenin signaling." in: Stem cells and development, Vol. 22, Issue 5, pp. 828-39, (2013) (PubMed).

Target Details for PITX2

Target: PITX2 (Paired-Like Homeodomain 2 (PITX2))

Alternative Name: PITX2

Background:

Background Information: The PITX2 gene encodes a member of the RIEG/PITX homeobox family, which is in the bicoid class of homeodomain proteins. This protein acts as a transcription factor and regulates procollagen lysyl hydroxylase gene expression. Mutations in PITX2 are associated with Axenfeld-Rieger syndrome (ARS), iridogoniodysgenesis syndrome (IGDS), and sporadic cases of Peters anomaly. This protein is involved in the development of the eye, tooth and abdominal organs. It also acts as a transcriptional regulator involved in basal and hormone-regulated activity of prolactin.

Gene Name: Paired-like homeodomain 2

Alternative Symbols: RS, RGS, ARP1, Brx1, IDG2, IGDS, IHG2, PTX2, RIEG, ASGD4, IGDS2, IRID2, Otlx2, RIEG1

Protein Name: Pituitary homeobox 2

Molecular Weight: 36kDa

Gene ID: 5308

NCBI Accession: NP_000316

UniProt: Q99697

Pathways: Retinoic Acid Receptor Signaling Pathway, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development