EED antibody (N-Term)

Catalog No. ABIN2776407

This anti-EED antibody is a Rabbit Polyclonal antibody detecting EED in WB and IF. Suitable for Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Zebrafish (Danio rerio) and Rabbit. This Primary Antibody has been cited in 2+ publications.

Quick Overview for EED antibody (N-Term) (ABIN2776407)

Target: EED (Embryonic Ectoderm Development (EED))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Zebrafish (Danio rerio), Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EED antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-EED Antibody

Binding Specificity: N-Term

Sequence: GDENDDAVSI ESGTNTERPD TPTNTPNAPG RKSWGKGKWK SKKCKYSFKC

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 100%, Zebrafish: 85%

Characteristics: This is a rabbit polyclonal antibody against EED. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human EED

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 441 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-EED antibody (ABIN2776407)

Lin, Huang, Chang, Chang, Yen, Lee, Chang, Liu, Chang: "Long noncoding RNA TUG1 is downregulated in non-small cell lung cancer and can regulate CELF1 on binding to PRC2." in: BMC cancer, Vol. 16, pp. 583, (2016) (PubMed).

Rakotobe, Violot, Hong, Gouet, Boulanger: "Mapping of immunogenic and protein-interacting regions at the surface of the seven-bladed beta-propeller domain of the HIV-1 cellular interactor EED." in: Virology journal, Vol. 5, pp. 32, (2008) (PubMed).

Target Details for EED

Target: EED (Embryonic Ectoderm Development (EED))

Alternative Name: EED

Background: EED is a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhance
Alias Symbols: HEED, WAIT1
Protein Interaction Partner: PINK1, BRCA1, ADAM17, EZH2, IMP3, PSPC1, NAT10, PBRM1, RIF1, RBM28, HEATR1, MAGOHB, ARGLU1, MRPL20, NRDE2, CASZ1, BCOR, EXOSC4, LUC7L3, SF3B6, NOP58, SRRT, RAB14, C9orf114, CRNKL1, DDX47, HP1BP3, MYEF2, TRA2A, CPSF1, IGHV3-23, UTP20, PRPF19, ZNF638, RBMX,
Protein Size: 441

Molecular Weight: 50 kDa

Gene ID: 8726

NCBI Accession: NM_003797, NP_003788

UniProt: O75530