KLF4 antibody (N-Term)

Catalog No. ABIN2776415

The Rabbit Polyclonal anti-KLF4 antibody is suitable to detect KLF4 in samples from Human, Cow, Horse, Dog, Sheep, Pig and Rabbit. It has been validated for WB.

Quick Overview for KLF4 antibody (N-Term) (ABIN2776415)

Target: KLF4 (Kruppel-Like Factor 4 (Gut) (KLF4))

Reactivity: Human, Cow, Horse, Dog, Sheep, Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KLF4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-KLF4 Antibody

Binding Specificity: N-Term

Purpose: KLF4 Antibody - N-terminal region

Sequence: GAACGGSNLA PLPRRETEEF NDLLDLDFIL SNSLTHPPES VAATVSSSAS

Predicted Reactivity: Cow: 80%, Dog: 87%, Horse: 87%, Human: 100%, Pig: 80%, Rabbit: 85%, Sheep: 80%

Characteristics: This is a rabbit polyclonal antibody against KLF4. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human KLF4

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8104053

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for KLF4

Target: KLF4 (Kruppel-Like Factor 4 (Gut) (KLF4))

Alternative Name: KLF4

Background:

Background Information: The mammalian Kruppel-like transcription factor, KLF4 is involved in preventing centrosome amplification following DNA damage caused by gamma-irradiation. It is both necessary and sufficient in preventing centrosome amplification following gamma-radiation-induced DNA damage and does so by transcriptionally suppressing cyclin E expression n. Kruppel-like factor 4 is also known to exhibit checkpoint function during the G1/S and G2/M transitions of the cell cycle.

Gene Name: Kruppel-like factor 4 (gut)

Alternative Symbols: EZF, GKLF

Protein Name: Krueppel-like factor 4

Molecular Weight: 50kDa

Gene ID: 9314

NCBI Accession: NP_004226

UniProt: O43474

Pathways: Peptide Hormone Metabolism, Stem Cell Maintenance