ILF2 antibody (C-Term)

Catalog No. ABIN2776419

This Rabbit Polyclonal antibody specifically detects ILF2 in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Dog, Cow, Rabbit, Horse, Guinea Pig and Zebrafish (Danio rerio) and has been mentioned in 2+ publications.

Quick Overview for ILF2 antibody (C-Term) (ABIN2776419)

Target: ILF2 (Interleukin Enhancer Binding Factor 2, 45kDa (ILF2))

Reactivity: Human, Mouse, Rat, Dog, Cow, Rabbit, Horse, Guinea Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ILF2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-ILF2 Antibody

Binding Specificity: C-Term

Sequence: HGGFRKILGQ EGDASYLASE ISTWDGVIVT PSEKAYEKPP EKKEGEEEEE

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against ILF2. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human ILF2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 390 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-ILF2 antibody (ABIN2776419)

Yamauchi, Nakamura, Hiramoto, Yuri, Yokota, Naitou, Takeuchi, Yamanaka, Kita, Nakahara, Kinoyama, Matsuhisa, Kaneko, Koutoku, Sasamata, Kobori, Katou, Tawara, Kawabata, Furuichi: "Sepantronium bromide (YM155) induces disruption of the ILF3/p54(nrb) complex, which is required for survivin expression." in: Biochemical and biophysical research communications, Vol. 425, Issue 4, pp. 711-6, (2012) (PubMed).

Jiang, Zhou, Moxley, Jarrett: "Purification and identification of positive regulators binding to a novel element in the c-Jun promoter." in: Biochemistry, Vol. 47, Issue 35, pp. 9318-34, (2008) (PubMed).

Target Details for ILF2

Target: ILF2 (Interleukin Enhancer Binding Factor 2, 45kDa (ILF2))

Alternative Name: ILF2

Background: Nuclear factor of activated T-cells (NFAT) is a transcription factor required for T-cell expression of the interleukin 2 gene. NFAT binds to a sequence in the interleukin 2 gene enhancer known as the antigen receptor response element 2. In addition, NFAT can bind RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. NFAT is a heterodimer of 45 kDa and 90 kDa proteins, the smaller of which is the product of the ILF2 gene. The encoded protein binds strongly to the 90 kDa protein and stimulates its ability to enhance gene expression.
Alias Symbols: NF45, PRO3063
Protein Interaction Partner: CCNDBP1, HUWE1, UBC, EEF1G, AURKA, SUMO2, SUMO3, STAU1, IVNS1ABP, RPA1, SMURF2, RPA3, RPA2, ERG, EZH2, BMI1, SUZ12, EED, rev, HNRNPU, FKBP3, DHX9, DDX1, ABCF1, C14orf166, RTCB, DIMT1, NELFB, EDC4, IGF2BP3, EIF2B2, EIF2B3, YBX3, RFC4, PTBP1, YBX1, NMT1, MR
Protein Size: 390

Molecular Weight: 43 kDa

Gene ID: 3608

NCBI Accession: NM_004515, NP_004506

UniProt: Q12905