HMGB3 antibody (C-Term)

Catalog No. ABIN2776438

The Rabbit Polyclonal anti-HMGB3 antibody (ABIN2776438) specifically detects HMGB3 in WB and IHC. The antibody is reactive with Human, Mouse, Rat, Cow, Dog, Horse, Rabbit, Guinea Pig and Zebrafish (Danio rerio) samples.

Quick Overview for HMGB3 antibody (C-Term) (ABIN2776438)

Target: HMGB3 (High Mobility Group Box 3 (HMGB3))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Rabbit, Guinea Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HMGB3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-HMGB3 Antibody

Binding Specificity: C-Term

Purpose: HMGB3 Antibody - C-terminal region

Sequence: NLNDSEKQPY ITKAAKLKEK YEKDVADYKS KGKFDGAKGP AKVARKKVEE

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 77%

Characteristics: This is a rabbit polyclonal antibody against HMGB3. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human HMGB3

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8102518

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for HMGB3

Target: HMGB3 (High Mobility Group Box 3 (HMGB3))

Alternative Name: HMGB3

Background:

Background Information: The human HMG2a gene is transcribed mainly in the placenta. HMG2a encodes a protein which is part of the high mobility group (HMG) family. Members of this family are ubiquitously expressed and facilitate the formation of nucleoprotein complexes where the DNA is sharply bent.

Gene Name: High mobility group box 3

Alternative Symbols: HMG4, HMG-4, HMG2A, HMG-2a

Protein Name: High mobility group protein B3

Molecular Weight: 23kDa

Gene ID: 3149

NCBI Accession: NP_005333

UniProt: O15347