POLR2I antibody (N-Term)

Catalog No. ABIN2776452

The Rabbit Polyclonal anti-POLR2I antibody has been validated for WB. It is suitable to detect POLR2I in samples from Human, Mouse, Rat, Dog, Rabbit, Cow, Guinea Pig, Horse, Zebrafish (Danio rerio) and Saccharomyces cerevisiae.

Quick Overview for POLR2I antibody (N-Term) (ABIN2776452)

Target: POLR2I (Polymerase (RNA) II (DNA Directed) Polypeptide I, 14.5kDa (POLR2I))

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Cow, Guinea Pig, Horse, Zebrafish (Danio rerio), Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR2I antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-POLR2I Antibody

Binding Specificity: N-Term

Sequence: ACRNCDYQQE ADNSCIYVNK ITHEVDELTQ IIADVSQDPT LPRTEDHPCQ

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 83%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against POLR2I. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human POLR2I

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 125 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for POLR2I

Target: POLR2I (Polymerase (RNA) II (DNA Directed) Polypeptide I, 14.5kDa (POLR2I))

Alternative Name: POLR2I

Background: POLR2I is a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with two other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. POLR2I has two zinc finger motifs with conserved cysteines and the subunit does possess zinc binding activity. Western blots using two different antibodies against two unique regions of this protein target confirm the same apparent molecular weight in our tests.This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with two other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. The product of this gene has two zinc finger motifs with conserved cysteines and the subunit does possess zinc binding activity. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: RPB9, hRPB14.5
Protein Interaction Partner: ESR1, TCEB3, SRC, PSMB9, UBC, MED19, MED26, RECQL5, TBCD, BRCA1, CPSF6, POLR2E, POLR2D, POLR2C, POLR2B, POLR2A, DCUN1D1, CUL5, MYC, tat, WDR48, POLR2H, MED10, MED29,
Protein Size: 125

Molecular Weight: 14 kDa

Gene ID: 5438

NCBI Accession: NM_006233, NP_006224

UniProt: P36954

Pathways: Regulatory RNA Pathways