POLR2I antibody

Catalog No. ABIN7231152

The Rabbit Polyclonal anti-POLR2I antibody (ABIN7231152) specifically detects POLR2I in WB and ELISA. The antibody is reactive with Human and Mouse samples.

Quick Overview for POLR2I antibody (ABIN7231152)

Target: POLR2I (Polymerase (RNA) II (DNA Directed) Polypeptide I, 14.5kDa (POLR2I))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR2I antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-POLR2I Antibody

Purpose: Rabbit Anti-RPB9 Polyclonal Antibody

Specificity: The antibody detects endogenous levels of RPB9 protein

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human RPB9 protein

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 %BSA

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for POLR2I

Target: POLR2I (Polymerase (RNA) II (DNA Directed) Polypeptide I, 14.5kDa (POLR2I))

Alternative Name: RPB9

Background: DNA-directed RNA polymerase II subunit RPB9, DNA-directed RNA polymerase II subunit I, RNA polymerase II 14.5 kDa subunitPOLR2I encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with two other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. The product of this gene has two zinc finger motifs with conserved cysteines and the subunit does possess zinc binding activity. POLR2I (RNA Polymerase II Subunit I) is a Protein Coding gene. Diseases associated with POLR2I include Hiv-1. Among its related pathways are Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3 and DNA damage_Role of Brca1 and Brca2 in DNA repair.

Molecular Weight: 13kD

Gene ID: 5438

UniProt: P36954

Pathways: Regulatory RNA Pathways