Nucleolar Protein 6 antibody (N-Term)

Catalog No. ABIN2776561

The Rabbit Polyclonal anti-Nucleolar Protein 6 antibody is suitable to detect Nucleolar Protein 6 in samples from Human, Mouse, Rat, Guinea Pig, Cow, Dog, Horse, Rabbit and Saccharomyces cerevisiae. It has been validated for WB.

Quick Overview for Nucleolar Protein 6 antibody (N-Term) (ABIN2776561)

Target: Nucleolar Protein 6 (NOL6)

Reactivity: Human, Mouse, Rat, Guinea Pig, Cow, Dog, Horse, Rabbit, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nucleolar Protein 6 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Nucleolar Protein 6 Antibody

Binding Specificity: N-Term

Purpose: NOL6 Antibody - N-terminal region

Sequence: SRKRTLAEPP AKGLLQPVKL SRAELYKEPT NEELNRLRET EILFHSSLLR

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 100%, Yeast: 90%

Characteristics: This is a rabbit polyclonal antibody against NOL6. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human NOL6

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP40994-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for Nucleolar Protein 6

Target: Nucleolar Protein 6 (NOL6)

Alternative Name: NOL6

Background:

Background Information: NOL6 is a nucleolar RNA-associated protein that is highly conserved between species. RNase treatment of permeabilized cells indicates that the nucleolar localization is RNA dependent. Further studies suggest that the protein is associated with ribosome biogenesis through an interaction with pre-rRNA primary transcripts. The nucleolus is a dense subnuclear membraneless organelle that assembles around clusters of rRNA genes and functions in ribosome biogenesis. This gene encodes a nucleolar RNA-associated protein that is highly conserved between species. RNase treatment of permeabilized cells indicates that the nucleolar localization is RNA dependent. Further studies suggest that the protein is associated with ribosome biogenesis through an interaction with pre-rRNA primary transcripts. Alternative splicing has been observed at this locus and two splice variants encoding distinct isoforms have been identified.

Gene Name: Nucleolar protein family 6 (RNA-associated)

Alternative Symbols: NRAP, UTP22, bA311H10.1

Protein Name: Nucleolar protein 6

Molecular Weight: 127kDa

Gene ID: 65083

NCBI Accession: NP_075068

UniProt: Q9H6R4