Phosphatidylinositol Glycan Anchor Biosynthesis, Class Z (PIGZ) (N-Term) antibody

Details for Product No. ABIN2776579
Antigen
Epitope
N-Term
4
1
Reactivity
Cow (Bovine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Yeast (Saccharomyces cerevisiae)
10
3
3
1
1
1
1
1
1
1
Host
Rabbit
10
Clonality
Polyclonal
Conjugate
Un-conjugated
1
1
1
Application
Western Blotting (WB)
8
6
1
1
Options
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human PIGZ
Blocking Peptide Blocking peptide for this product available: ABIN981757
Sequence VLWGGLSLLR VLWCLLPQTG YVHPDEFFQS PEVMAEDILG VQAARPWEFY
Predicted Reactivity Cow: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 93%, Rabbit: 100%, Rat: 100%, Yeast: 80%
Characteristics This is a rabbit polyclonal antibody against PIGZ. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Antigen
Alternative Name PIGZ (PIGZ Antibody Abstract)
Background The glycosylphosphatidylinositol (GPI) anchor is a glycolipid found on many blood cells that serves to anchor proteins to the cell surface. PIGZ is a protein that is localized to the endoplasmic reticulum, and is involved in GPI anchor biosynthesis. As shown for the yeast homolog, which is a member of a family of dolichol-phosphate-mannose (Dol-P-Man)-dependent mannosyltransferases, this protein can also add a side-branching fourth mannose to GPI precursors during the assembly of GPI anchors.The glycosylphosphatidylinositol (GPI) anchor is a glycolipid found on many blood cells that serves to anchor proteins to the cell surface. This gene encodes a protein that is localized to the endoplasmic reticulum, and is involved in GPI anchor biosynthesis. As shown for the yeast homolog, which is a member of a family of dolichol-phosphate-mannose (Dol-P-Man)-dependent mannosyltransferases, this protein can also add a side-branching fourth mannose to GPI precursors during the assembly of GPI anchors.
Alias Symbols: FLJ12768, GPI-MT-IV, MGC52163, SMP3, PIG-Z
Protein Size: 579
Molecular Weight 63 kDa
Gene ID 80235
NCBI Accession NM_025163, NP_079439
UniProt Q86VD9
Pathways Inositol Metabolic Process
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 579 AA

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Supplier Images
Image no. 1 for anti-Phosphatidylinositol Glycan Anchor Biosynthesis, Class Z (PIGZ) (N-Term) antibody (ABIN2776579) WB Suggested Anti-PIGZ Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:1562500 Pos...
Product cited in: Taron, Colussi, Wiedman, Orlean, Taron: "Human Smp3p adds a fourth mannose to yeast and human glycosylphosphatidylinositol precursors in vivo." in: The Journal of biological chemistry, Vol. 279, Issue 34, pp. 36083-92, 2004 (PubMed).