EIF2A antibody (C-Term)

Catalog No. ABIN2776588

This anti-EIF2A antibody is a Rabbit Polyclonal antibody detecting EIF2A in WB and IHC. Suitable for Human, Mouse, Rat, Dog, Horse, Rabbit, Cow, Goat, Guinea Pig and Zebrafish (Danio rerio). This Primary Antibody has been cited in 3+ publications.

Quick Overview for EIF2A antibody (C-Term) (ABIN2776588)

Target: EIF2A (Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A))

Reactivity: Human, Mouse, Rat, Dog, Horse, Rabbit, Cow, Goat, Guinea Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF2A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-EIF2A Antibody

Binding Specificity: C-Term

Sequence: DKSPDLAPTP APQSTPRNTV SQSISGDPEI DKKIKNLKKK LKAIEQLKEQ

Predicted Reactivity: Cow: 86%, Dog: 100%, Goat: 83%, Guinea Pig: 93%, Horse: 86%, Human: 100%, Mouse: 93%, Rabbit: 93%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against EIF2A. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human EIF2A

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 585 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-EIF2A antibody (ABIN2776588)

Feng, Chen, Chan, Chen: "Therapeutic Role of Protein Tyrosine Phosphatase 1B in Parkinson's Disease via Antineuroinflammation and Neuroprotection In Vitro and In Vivo." in: Parkinson's disease, Vol. 2020, pp. 8814236, (2020) (PubMed).

Xu, Liu, Caffaro, Hirschberg: "Inhibition of Golgi apparatus glycosylation causes endoplasmic reticulum stress and decreased protein synthesis." in: The Journal of biological chemistry, Vol. 285, Issue 32, pp. 24600-8, (2010) (PubMed).

Su, Wang, Baltzis, Qu, Wong, Koromilas: "Tyrosine phosphorylation acts as a molecular switch to full-scale activation of the eIF2alpha RNA-dependent protein kinase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 1, pp. 63-8, (2006) (PubMed).

Target Details for EIF2A

Target: EIF2A (Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A))

Alternative Name: EIF2A

Background: EIF2A is a 65-kD protein that catalyzes the formation of puromycin-sensitive 80S preinitiation complexes.EIF2A is a 65-kD protein that catalyzes the formation of puromycin-sensitive 80S preinitiation complexes (Zoll et al., 2002).[supplied by OMIM].
Alias Symbols: CDA02, MST089, MSTP004, MSTP089, EIF-2A
Protein Interaction Partner: UBC, CDC20, RPS23, RPS19, RPS16, RPS10, RPS7, RPS5, HDLBP, PRUNE2, EIF3K, SERBP1, RPS29, RPS24, EIF2AK2, MLH1, NXF1, ILF3, PA2G4, APP, PPP1CC, NEDD8, DCC, ATF4,
Protein Size: 585

Molecular Weight: 64 kDa

Gene ID: 83939

NCBI Accession: NM_032025, NP_114414

UniProt: Q9BY44

Pathways: Ribonucleoprotein Complex Subunit Organization, ER-Nucleus Signaling, Hepatitis C, Methionine Biosynthetic Process, Ribosome Assembly