EGF antibody (Middle Region)

Catalog No. ABIN2776844

The Rabbit Polyclonal anti-EGF antibody has been validated for WB. It is suitable to detect EGF in samples from Human, Mouse, Rat, Pig, Dog, Rabbit and Horse. There is 1 publication available.

Quick Overview for EGF antibody (Middle Region) (ABIN2776844)

Target: EGF (Epidermal Growth Factor (EGF))

Reactivity: Human, Mouse, Rat, Pig, Dog, Rabbit, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EGF antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-EGF Antibody

Binding Specificity: Middle Region

Sequence: ITIDFLTDKL YWCDAKQSVI EMANLDGSKR RRLTQNDVGH PFAVAVFEDY

Predicted Reactivity: Dog: 86%, Horse: 93%, Human: 100%, Mouse: 79%, Pig: 93%, Rabbit: 93%, Rat: 86%

Characteristics: This is a rabbit polyclonal antibody against EGF. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human EGF

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1207 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-EGF antibody (ABIN2776844)

Teixeira, Ribeiro, Cardoso, Pinto, Lobo, Fraga, Pina, Calais-da-Silva, Medeiros: "Genetic polymorphism in EGF is associated with prostate cancer aggressiveness and progression-free interval in androgen blockade-treated patients." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 14, Issue 11, pp. 3367-71, (2008) (PubMed).

Target Details for EGF

Target: EGF (Epidermal Growth Factor (EGF))

Alternative Name: EGF

Background: Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-
Alias Symbols: URG, HOMG4
Protein Interaction Partner: EGFR, PCGF2, UBE2I, SREBF1, LMNB1, EMD, LMNA, ZFYVE28, PIK3R2, ADAM12, DUSP3, VTN, ERBB3, ERBB2, NRG1, EGF, CPM, ADRBK1, GRB2, MAPKAPK2, RPS6KA5, FNBP1,
Protein Size: 1207

Molecular Weight: 6 kDa

Gene ID: 1950

NCBI Accession: NM_001963, NP_001954

UniProt: P01133

Pathways: NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation