EGF antibody (Middle Region)

Catalog No. ABIN2776844

There is 1 publication for this product available. The Rabbit Polyclonal anti-EGF antibody is suitable to detect EGF in samples from Human, Mouse, Rat, Dog, Pig, Rabbit and Horse. It has been validated for WB.

Quick Overview for EGF antibody (Middle Region) (ABIN2776844)

Target: EGF (Epidermal Growth Factor (EGF))

Reactivity: Human, Mouse, Rat, Dog, Pig, Rabbit, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EGF antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-EGF Antibody

Binding Specificity: Middle Region

Sequence: ITIDFLTDKL YWCDAKQSVI EMANLDGSKR RRLTQNDVGH PFAVAVFEDY

Predicted Reactivity: Dog: 86%, Horse: 93%, Human: 100%, Mouse: 79%, Pig: 93%, Rabbit: 93%, Rat: 86%

Characteristics: This is a rabbit polyclonal antibody against EGF. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human EGF

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1207 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-EGF antibody (ABIN2776844)

Teixeira, Ribeiro, Cardoso, Pinto, Lobo, Fraga, Pina, Calais-da-Silva, Medeiros: "Genetic polymorphism in EGF is associated with prostate cancer aggressiveness and progression-free interval in androgen blockade-treated patients." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 14, Issue 11, pp. 3367-71, (2008) (PubMed).

Target Details for EGF

Target: EGF (Epidermal Growth Factor (EGF))

Alternative Name: EGF

Background: Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-
Alias Symbols: URG, HOMG4
Protein Interaction Partner: EGFR, PCGF2, UBE2I, SREBF1, LMNB1, EMD, LMNA, ZFYVE28, PIK3R2, ADAM12, DUSP3, VTN, ERBB3, ERBB2, NRG1, EGF, CPM, ADRBK1, GRB2, MAPKAPK2, RPS6KA5, FNBP1,
Protein Size: 1207

Molecular Weight: 6 kDa

Gene ID: 1950

NCBI Accession: NM_001963, NP_001954

UniProt: P01133

Pathways: NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation