ASL antibody (N-Term)

Catalog No. ABIN2776927

There is 1 publication for this product available. The Rabbit Polyclonal anti-ASL antibody is suitable to detect ASL in samples from Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Guinea Pig, Horse, Rabbit, Dog, Goat and Saccharomyces cerevisiae. It has been validated for WB.

Quick Overview for ASL antibody (N-Term) (ABIN2776927)

Target: ASL (Argininosuccinate Lyase (ASL))

Reactivity: Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Guinea Pig, Horse, Rabbit, Dog, Goat, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ASL antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ASL Antibody

Binding Specificity: N-Term

Purpose: ASL Antibody - N-terminal region

Sequence: GATAGKLHTG RSRNDQVVTD LRLWMRQTCS TLSGLLWELI RTMVDRAEAE

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against ASL. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ASL

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8094939

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-ASL antibody (ABIN2776927)

Trevisson, Salviati, Baldoin, Toldo, Casarin, Sacconi, Cesaro, Basso, Burlina: "Argininosuccinate lyase deficiency: mutational spectrum in Italian patients and identification of a novel ASL pseudogene." in: Human mutation, Vol. 28, Issue 7, pp. 694-702, (2007) (PubMed).

Target Details for ASL

Target: ASL (Argininosuccinate Lyase (ASL))

Alternative Name: ASL

Background:

Background Information: ASL is a member of the lyase 1 family. The protein forms a cytosolic homotetramer and primarily catalyzes the reversible hydrolytic cleavage of argininosuccinate into arginine and fumarate, an essential step in the liver in detoxifying ammonia via the urea cycle. Mutations in its gene result in the autosomal recessive disorder argininosuccinic aciduria, or argininosuccinic acid lyase deficiency.This gene encodes a member of the lyase 1 family. The encoded protein forms a cytosolic homotetramer and primarily catalyzes the reversible hydrolytic cleavage of argininosuccinate into arginine and fumarate, an essential step in the liver in detoxifying ammonia via the urea cycle. Mutations in this gene result in the autosomal recessive disorder argininosuccinic aciduria, or argininosuccinic acid lyase deficiency. A nontranscribed pseudogene is also located on the long arm of chromosome 22. Alternatively spliced transcript variants encoding different isoforms have been described.

Gene Name: Argininosuccinate lyase

Alternative Symbols: ASAL

Protein Name: Argininosuccinate lyase

Molecular Weight: 52kDa

Gene ID: 435

NCBI Accession: NP_000039

UniProt: P04424

Pathways: Response to Growth Hormone Stimulus