Keratin 10 antibody (N-Term)

Catalog No. ABIN2776960

Cited in 1 publication. The Rabbit Polyclonal anti-Keratin 10 antibody (ABIN2776960) specifically detects Keratin 10 in WB. The antibody is reactive with Human, Mouse, Rat, Dog, Rabbit, Cow, Pig and Horse samples.

Quick Overview for Keratin 10 antibody (N-Term) (ABIN2776960)

Target: Keratin 10 (KRT10)

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Cow, Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Keratin 10 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Keratin 10 Antibody

Binding Specificity: N-Term

Purpose: KRT10 Antibody - N-terminal region

Sequence: EKVTMQNLND RLASYLDKVR ALEESNYELE GKIKEWYEKH GNSHQGEPRD

Predicted Reactivity: Cow: 93%, Dog: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 86%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against KRT10. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human KRT10

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP41730-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-Keratin 10 antibody (ABIN2776960)

Tsubota, Akiyama, Kanitakis, Sakai, Nomura, Claudy, Shimizu: "Mild recessive bullous congenital ichthyosiform erythroderma due to a previously unidentified homozygous keratin 10 nonsense mutation." in: The Journal of investigative dermatology, Vol. 128, Issue 7, pp. 1648-52, (2008) (PubMed).

Target Details for Keratin 10

Target: Keratin 10 (KRT10)

Alternative Name: KRT10

Background:

Background Information: KRT10 is a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in the gene encoding KRT10 are associated with epidermolytic hyperkeratosis. This gene encodes a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis. This gene is located within a cluster of keratin family members on chromosome 17q21. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Gene Name: Keratin 10

Alternative Symbols: BIE, EHK, K10, KPP, BCIE, CK10

Protein Name: Keratin, type I cytoskeletal 10

Molecular Weight: 59kDa

Gene ID: 3858

NCBI Accession: NP_000412

UniProt: P13645