Prothrombin antibody (Middle Region)

Catalog No. ABIN2776976

This Rabbit Polyclonal antibody specifically detects Prothrombin in WB and IHC. It exhibits reactivity toward Human, Rat, Mouse, Cow, Dog, Guinea Pig, Horse, Sheep and Zebrafish (Danio rerio).

Quick Overview for Prothrombin antibody (Middle Region) (ABIN2776976)

Target: Prothrombin (F2) (Coagulation Factor II (thrombin) (F2))

Reactivity: Human, Rat, Mouse, Cow, Dog, Guinea Pig, Horse, Sheep, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Prothrombin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Prothrombin Antibody

Binding Specificity: Middle Region

Sequence: ISDRWVLTAA HCLLYPPWDK NFTENDLLVR IGKHSRTRYE RNIEKISMLE

Predicted Reactivity: Cow: 92%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rat: 100%, Sheep: 93%, Zebrafish: 77%

Characteristics: This is a rabbit polyclonal antibody against F2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human F2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 622 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for Prothrombin

Target: Prothrombin (F2) (Coagulation Factor II (thrombin) (F2))

Alternative Name: F2

Background: Coagulation factor II is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. F2 also plays a role in maintaining vascular integrity during development and postnatal life.Coagulation factor II is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. F2 also plays a role in maintaining vascular integrity during development and postnatal life. Mutations in F2 leads to various forms of thrombosis and dysprothrombinemia. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: PT
Protein Interaction Partner: ST13, FN1, DDIT3, ALB, F11, PROZ, ITGA2B, SERPING1, HGFAC, AKR7A2, CPB2, F2RL3, F2RL2, SERPINA5, SERPINB8, AANAT, F2RL1, F9, F8, GP1BA, KNG1, F5, C6, PLAU, THBS1, THBD, F2R, SERPINE2, F2, PROS1, AMBP, PROC, GP5, SERPINE1, SERPINB6, SPP1, IGFBP5, IGFBP3, S
Protein Size: 622

Molecular Weight: 70 kDa

Gene ID: 2147

NCBI Accession: NM_000506, NP_000497

UniProt: P00734

Pathways: Complement System, Peptide Hormone Metabolism, Regulation of G-Protein Coupled Receptor Protein Signaling