DHODH antibody (N-Term)

Catalog No. ABIN2777041

There is 1 publication for this product available. The Rabbit Polyclonal anti-DHODH antibody is suitable to detect DHODH in samples from Human, Mouse, Rat, Dog, Horse, Zebrafish (Danio rerio), Cow, Rabbit, Guinea Pig, Pig, Saccharomyces cerevisiae and Goat. It has been validated for WB.

Quick Overview for DHODH antibody (N-Term) (ABIN2777041)

Target: DHODH (Dihydroorotate Dehydrogenase (DHODH))

Reactivity: Human, Mouse, Rat, Dog, Horse, Zebrafish (Danio rerio), Cow, Rabbit, Guinea Pig, Pig, Saccharomyces cerevisiae, Goat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DHODH antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-DHODH Antibody

Binding Specificity: N-Term

Purpose: DHODH Antibody - N-terminal region

Sequence: GEAVDGLYKM GFGFVEIGSV TPKPQEGNPR PRVFRLPEDQ AVINRYGFNS

Predicted Reactivity: Cow: 93%, Dog: 100%, Goat: 86%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 93%, Pig: 100%, Rabbit: 93%, Rat: 93%, Yeast: 86%, Zebrafish: 85%

Characteristics: This is a rabbit polyclonal antibody against DHODH. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human DHODH

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8098901

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-DHODH antibody (ABIN2777041)

Lazrek, Goffard, Schanen, Karquel, Bocket, Lion, Devaux, Hedouin, Gosset, Hober: "Detection of hepatitis C virus antibodies and RNA among medicolegal autopsy cases in Northern France." in: Diagnostic microbiology and infectious disease, Vol. 55, Issue 1, pp. 55-8, (2006) (PubMed).

Target Details for DHODH

Target: DHODH (Dihydroorotate Dehydrogenase (DHODH))

Alternative Name: DHODH

Background:

Background Information: DHODH catalyzes the fourth enzymatic step, the ubiquinone-mediated oxidation of dihydroorotate to orotate, in de novo pyrimidine biosynthesis. This protein is a mitochondrial protein located on the outer surface of the inner mitochondrial membrane.The protein encoded by this gene catalyzes the fourth enzymatic step, the ubiquinone-mediated oxidation of dihydroorotate to orotate, in de novo pyrimidine biosynthesis. This protein is a mitochondrial protein located on the outer surface of the inner mitochondrial membrane. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.The protein encoded by this gene catalyzes the fourth enzymatic step, the ubiquinone-mediated oxidation of dihydroorotate to orotate, in de novo pyrimidine biosynthesis. This protein is a mitochondrial protein located on the outer surface of the inner mitochondrial membrane. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Gene Name: Dihydroorotate dehydrogenase (quinone)

Alternative Symbols: URA1, POADS, DHOdehase

Protein Name: Dihydroorotate dehydrogenase (quinone), mitochondrial

Molecular Weight: 43kDa

Gene ID: 1723

NCBI Accession: NP_001352

UniProt: Q02127

Pathways: Ribonucleoside Biosynthetic Process, Protein targeting to Nucleus