Arachidonate 15-Lipoxygenase B (ALOX15B) (C-Term) antibody

Details for Product No. ABIN2777053
Target
Binding Specificity
C-Term
15
7
5
3
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat
56
23
8
5
5
4
4
2
2
1
Host
Rabbit
53
4
Clonality
Polyclonal
Conjugate
Un-conjugated
3
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Western Blotting (WB)
33
20
13
13
7
5
3
2
1
1
1
Options
Immunogen The immunogen is a synthetic peptide directed towards the C terminal region of human ALOX15B
Sequence ATCEGFIATL PPVNATCDVI LALWLLSKEP GDQRPLGTYP DEHFTEEAPR
Predicted Reactivity Cow: 93%, Dog: 93%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 93%, Rabbit: 93%, Rat: 93%
Characteristics This is a rabbit polyclonal antibody against ALOX15B. It was validated on Western Blot using a cell lysate as a positive control.
Purification Protein A purified
Target
Alternative Name ALOX15B (ALOX15B Antibody Abstract)
Background ALOX15B is a member of the lipoxygenase family of structurally related nonheme iron dioxygenases involved in the production of fatty acid hydroperoxides. The protein converts arachidonic acid exclusively to 15S-hydroperoxyeicosatetraenoic acid, while metabolizing linoleic acid less effectively.This gene encodes a member of the lipoxygenase family of structurally related nonheme iron dioxygenases involved in the production of fatty acid hydroperoxides. The encoded protein converts arachidonic acid exclusively to 15S-hydroperoxyeicosatetraenoic acid, while metabolizing linoleic acid less effectively. This gene is located in a cluster of related genes and a pseudogene that spans approximately 100 kilobases on the short arm of chromosome 17. Alternatively spliced transcript variants encoding different isoforms have been described.
Alias Symbols: 15-LOX-2
Protein Interaction Partner: RXRA, PPARG, ERAL1,
Protein Size: 676
Molecular Weight 74 kDa
Gene ID 247
NCBI Accession NM_001141, NP_001132
UniProt O15296
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 676 AA

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Images
Image no. 1 for anti-Arachidonate 15-Lipoxygenase B (ALOX15B) (C-Term) antibody (ABIN2777053) WB Suggested Anti-ALOX15B Antibody Titration: 2.5ug/ml Positive Control: Jurkat ce...
Product cited in: Subbarayan, Xu, Kim, Yang, Hoque, Sabichi, Llansa, Mendoza, Logothetis, Newman, Lippman, Menter: "Inverse relationship between 15-lipoxygenase-2 and PPAR-gamma gene expression in normal epithelia compared with tumor epithelia." in: Neoplasia (New York, N.Y.), Vol. 7, Issue 3, pp. 280-93, 2005 (PubMed).