TNNT1 antibody (Middle Region)

Catalog No. ABIN2777153

Product Details anti-TNNT1 Antibody

Target: TNNT1 (Slow Skeletal Troponin T (TNNT1))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TNNT1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: WIHQLESEKF DLMAKLKQQK YEINVLYNRI SHAQKFRKGA GKGRVGGRWK

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rat: 100%, Zebrafish: 85%

Characteristics: This is a rabbit polyclonal antibody against TNNT1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human TNNT1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 278 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for TNNT1

Target: TNNT1 (Slow Skeletal Troponin T (TNNT1))

Alternative Name: TNNT1 (TNNT1 Products)

Background: TNNT1 is a protein that is a subunit of troponin, which is a regulatory complex located on the thin filament of the sarcomere. This complex regulates striated muscle contraction in response to fluctuations in intracellular calcium concentration. This complex is composed of three subunits: troponin C, which binds calcium, troponin T, which binds tropomyosin, and troponin I, which is an inhibitory subunit. This protein is the slow skeletal troponin T subunit. Mutations in this gene cause nemaline myopathy type 5, also known as Amish nemaline myopathy, a neuromuscular disorder characterized by muscle weakness and rod-shaped, or nemaline, inclusions in skeletal muscle fibers which affects infants, resulting in death due to respiratory insufficiency, usually in the second year.This gene encodes a protein that is a subunit of troponin, which is a regulatory complex located on the thin filament of the sarcomere. This complex regulates striated muscle contraction in response to fluctuations in intracellular calcium concentration. This complex is composed of three subunits: troponin C, which binds calcium, troponin T, which binds tropomyosin, and troponin I, which is an inhibitory subunit. This protein is the slow skeletal troponin T subunit. Mutations in this gene cause nemaline myopathy type 5, also known as Amish nemaline myopathy, a neuromuscular disorder characterized by muscle weakness and rod-shaped, or nemaline, inclusions in skeletal muscle fibers which affects infants, resulting in death due to respiratory insufficiency, usually in the second year. Multiple transcript variants encoding different isoforms have been found for this gene.
Alias Symbols: ANM, MGC104241, TNT, STNT, TNTS
Protein Interaction Partner: CCDC136, TFIP11, LDOC1, MORF4L1, TBPL1, TPM3, TPM1, TNNT1, NFE2L2, KRT40, UBC, PI4KA, SERPINA4, OSM, MARS, HSP90AB1, FYN, EEF1G, DDX5, CHD3, BLOC1S2, C2orf44, ZNF768, ZNF250, ZC3H15, NAGK, HMP19, TRA2A, TMEM98, ARMC8, OSBP2, ZKSCAN5, LARP1, NACAD, SNW1, S
Protein Size: 278

Molecular Weight: 33 kDa

Gene ID: 7138

NCBI Accession: NM_003283, NP_003274

UniProt: P13805