TNNT1 antibody

Catalog No. ABIN7231858

The Rabbit Polyclonal anti-TNNT1 antibody is suitable to detect TNNT1 in samples from Human, Mouse and Rat. It has been validated for WB and ELISA.

Quick Overview for TNNT1 antibody (ABIN7231858)

Target: TNNT1 (Slow Skeletal Troponin T (TNNT1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TNNT1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-TNNT1 Antibody

Purpose: Rabbit Anti-TNNT1 Polyclonal Antibody

Specificity: The antibody detects endogenous levels of TNNT1 protein

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human TNNT1 protein

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 %BSA

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for TNNT1

Target: TNNT1 (Slow Skeletal Troponin T (TNNT1))

Alternative Name: TNNT1

Background: Troponin T, slow skeletal muscle, TnTs, Slow skeletal muscle troponin T, sTnTTNNT1 (Troponin T1, Slow Skeletal Type) encodes a protein that is a subunit of troponin, which is a regulatory complex located on the thin filament of the sarcomere. This complex regulates striated muscle contraction in response to fluctuations in intracellular calcium concentration. This complex is composed of three subunits: troponin C, which binds calcium, troponin T, which binds tropomyosin, and troponin I, which is an inhibitory subunit. This protein is the slow skeletal troponin T subunit. Mutations in TNNT1 cause nemaline myopathy type 5, also known as Amish nemaline myopathy, a neuromuscular disorder characterized by muscle weakness and rod-shaped, or nemaline, inclusions in skeletal muscle fibers which affects infants, resulting in death due to respiratory insufficiency, usually in the second year. Multiple transcript variants encoding different isoforms have been found for TNNT1.

Molecular Weight: 30kD

Gene ID: 7138

UniProt: P13805