ZEB1 antibody (N-Term)

Catalog No. ABIN2777193

Cited in 2+ publications. The Rabbit Polyclonal anti-ZEB1 antibody (ABIN2777193) specifically detects ZEB1 in WB and IHC. The antibody is reactive with Human, Mouse, Rat, Dog, Rabbit, Cow, Pig and Zebrafish (Danio rerio) samples.

Quick Overview for ZEB1 antibody (N-Term) (ABIN2777193)

Target: ZEB1 (Zinc Finger E-Box Binding Homeobox 1 (ZEB1))

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Cow, Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZEB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-ZEB1 Antibody

Binding Specificity: N-Term

Purpose: ZEB1 Antibody - N-terminal region

Sequence: NPRRNNVTNY NTVVETNSDS DDEDKLHIVE EESVTDAADC EGVPEDDLPT

Predicted Reactivity: Cow: 100%, Dog: 100%, Human: 100%, Mouse: 93%, Pig: 100%, Rabbit: 100%, Rat: 93%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against ZEB1. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ZEB1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP30976-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-ZEB1 antibody (ABIN2777193)

Kothandaraman, Bajic, Brendan, Huak, Keow, Razvi, Salto-Tellez, Choolani: "E2F5 status significantly improves malignancy diagnosis of epithelial ovarian cancer." in: BMC cancer, Vol. 10, pp. 64, (2010) (PubMed).

Li, Xia, Huang, Chen, Su, Li, Wang, Ding, Shao: "A strategy to rapidly identify the functional targets of microRNAs by combining bioinformatics and mRNA cytoplasmic/nucleic ratios in culture cells." in: FEBS letters, Vol. 584, Issue 14, pp. 3198-202, (2010) (PubMed).

Target Details for ZEB1

Target: ZEB1 (Zinc Finger E-Box Binding Homeobox 1 (ZEB1))

Alternative Name: ZEB1

Background:

Background Information: ZEB1 is a zinc finger transcription factor that represses T-lymphocyte-specific IL2 gene expression by binding to a negative regulatory domain 100 nucleotides 5-prime of the IL2 transcription start site.

Gene Name: Zinc finger E-box binding homeobox 1

Alternative Symbols: BZP, TCF8, AREB6, FECD6, NIL2A, PPCD3, ZFHEP, ZFHX1A, DELTAEF1

Protein Name: Zinc finger E-box-binding homeobox 1

Molecular Weight: 124kDa

Gene ID: 6935

NCBI Accession: NP_110378

UniProt: P37275

Pathways: Regulation of Muscle Cell Differentiation