NFYB antibody (Middle Region)

Catalog No. ABIN2777242

This Rabbit Polyclonal antibody specifically detects NFYB in WB. It exhibits reactivity toward Human, Mouse, Rat, Dog, Cow, Pig, Guinea Pig, Rabbit, Horse and Saccharomyces cerevisiae.

Quick Overview for NFYB antibody (Middle Region) (ABIN2777242)

Target: NFYB (Nuclear Transcription Factor Y, beta (NFYB))

Reactivity: Human, Mouse, Rat, Dog, Cow, Pig, Guinea Pig, Rabbit, Horse, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFYB antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NFYB Antibody

Binding Specificity: Middle Region

Purpose: NFYB Antibody - middle region

Sequence: EAMKGEKGIG GAVTATDGLS EELTEEAFTN QLPAGLITTD GQQQNVMVYT

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 86%, Horse: 93%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Yeast: 91%

Characteristics: This is a rabbit polyclonal antibody against NFYB. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human NFYB

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP31262-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for NFYB

Target: NFYB (Nuclear Transcription Factor Y, beta (NFYB))

Alternative Name: NFYB

Background:

Background Information: NFYB is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence, protein sequence alignments and experiments with mutants.

Gene Name: Nuclear transcription factor Y, beta

Alternative Symbols: HAP3, CBF-A, CBF-B, NF-YB

Protein Name: Nuclear transcription factor Y subunit beta

Molecular Weight: 23kDa

Gene ID: 4801

NCBI Accession: NP_006157

UniProt: P25208

Pathways: Regulation of Lipid Metabolism by PPARalpha