MED16 antibody (N-Term)

Catalog No. ABIN2777307

The Rabbit Polyclonal anti-MED16 antibody (ABIN2777307) specifically detects MED16 in WB. The antibody is reactive with Human, Mouse, Rat, Dog, Cow, Guinea Pig and Horse samples.

Quick Overview for MED16 antibody (N-Term) (ABIN2777307)

Target: MED16 (Mediator Complex Subunit 16 (MED16))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED16 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MED16 Antibody

Binding Specificity: N-Term

Purpose: MED16 Antibody - N-terminal region

Sequence: FGGKPMEGWI AVTVSGLVTV SLLKPSGQVL TSTESLCRLR GRVALADIAF

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against MED16. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human MED16

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8104989

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for MED16

Target: MED16 (Mediator Complex Subunit 16 (MED16))

Alternative Name: MED16

Background:

Targeted Subunit: 16

Background Information: THRAP5 is part of the human thyroid hormone receptor-associated protein (TRAP)-Mediator family which acts as a coactivator for a broad range of nuclear hormone receptors as well as other classes of transcriptional activators.

Gene Name: Mediator complex subunit 16

Alternative Symbols: DRIP92, THRAP5, TRAP95

Protein Name: Mediator of RNA polymerase II transcription subunit 16

Molecular Weight: 97kDa

Gene ID: 10025

NCBI Accession: NP_005472

UniProt: Q9Y2X0

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Regulation of Lipid Metabolism by PPARalpha