KAP1 antibody (N-Term)

Catalog No. ABIN2777312

This Rabbit Polyclonal antibody specifically detects KAP1 in WB. It exhibits reactivity toward Human, Mouse, Rat, Dog and Cow.

Quick Overview for KAP1 antibody (N-Term) (ABIN2777312)

Target: KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))

Reactivity: Human, Mouse, Rat, Dog, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KAP1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-KAP1 Antibody

Binding Specificity: N-Term

Sequence: AASAAAASAA AASAASGSPG PGEGSAGGEK RSTAPSAAAS ASASAAASSP

Predicted Reactivity: Cow: 92%, Dog: 100%, Human: 100%, Mouse: 93%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against TRIM28. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human TRIM28

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 835 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for KAP1

Target: KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))

Alternative Name: TRIM28

Background: TRIM28 mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. TRIM28 localizes to the nucleus and is thought to associate with specific chromatin regions. It is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING,a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: FLJ29029, KAP1, RNF96, TF1B, TIF1B
Protein Interaction Partner: WWOX, ERLIN2, HUWE1, SUMO1, UBC, SPRTN, SENP7, HAUS2, MAGEC2, CBX5, WHSC1, UBE2I, AURKA, SUMO2, SUMO3, RNF4, CEP250, TRIM28, HIF1A, CHD3, ATM, ZNF350, STAU1, HIST3H3, LYN, NEDD8, MDM2, ZBTB1, RPA3, RPA2, RPA1, RNF2, SUZ12, EZH2, BMI1, EED, BRCC3, WRNIP1,
Protein Size: 835

Molecular Weight: 88 kDa

Gene ID: 10155

NCBI Accession: NM_005762, NP_005753

UniProt: Q13263

Pathways: Hedgehog Signaling, Positive Regulation of Response to DNA Damage Stimulus