MBD1 antibody (C-Term)

Catalog No. ABIN2777532

Product Details anti-MBD1 Antibody

Target: MBD1 (Methyl-CpG Binding Domain Protein 1 (MBD1))

Binding Specificity: C-Term

Reactivity: Human, Rat, Dog, Cow, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MBD1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: EEDKEENKDD SASKLAPEEE AGGAGTPVIT EIFSLGGTRF RDTAVWLPRS

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against MBD1. It was validated on Western Blot.

Purification: Affinity Purified

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 629 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for MBD1

Target: MBD1 (Methyl-CpG Binding Domain Protein 1 (MBD1))

Alternative Name: MBD1 (MBD1 Products)

Synonyms: CXXC3 antibody, PCM1 antibody, RFT antibody, Cxxc3 antibody, CCDC11 antibody, methyl-CpG binding domain protein 1 antibody, MBD1 antibody, Mbd1 antibody

Background: The protein encoded by this gene is a member of a family of nuclear proteins related by the presence of a methyl-CpG binding domain (MBD). These proteins are capable of binding specifically to methylated DNA, and some members can also repress transcription from methylated gene promoters. This protein contains multiple domains: MBD at the N-terminus that functions both in binding to methylated DNA and in protein interactions, several CXXC-type zinc finger domains that mediate binding to non-methylated CpG dinucleotides, transcriptional repression domain (TRD) at the C-terminus that is involved in transcription repression and in protein interactions. Numerous alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
Alias Symbols: CXXC3, PCM1, RFT
Protein Interaction Partner: SUMO1, HTT, UBC, ECT2, APP, ATXN1L, TENM1, SUMO2, HIST1H3A, PCNA, ATF7IP2, ATF7IP, CHAF1A, SETDB1, PIAS1, STAT1, PRAM1, HDAC3, PIAS2, ZNF512B, Rnf2, CBX4, CBX5, HIPK3, SMAD9, OASL, SMAD5, SMAD3, SMAD7, SMAD1, SUV39H1,
Protein Size: 629

Molecular Weight: 69 kDa

Gene ID: 4152

NCBI Accession: NM_001204138, NP_001191067

UniProt: Q9UIS9