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NFIB antibody (Middle Region)

This Rabbit Polyclonal antibody specifically detects NFIB in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Sheep and Zebrafish (Danio rerio)and has been mentioned in 1 publication.
Catalog No. ABIN2777544

Quick Overview for NFIB antibody (Middle Region) (ABIN2777544)

Target

See all NFIB Antibodies
NFIB (Nuclear Factor I/B (NFIB))

Reactivity

  • 33
  • 10
  • 7
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Sheep, Zebrafish (Danio rerio)

Host

  • 26
  • 9
Rabbit

Clonality

  • 25
  • 10
Polyclonal

Conjugate

  • 30
  • 1
  • 1
  • 1
  • 1
  • 1
This NFIB antibody is un-conjugated

Application

  • 35
  • 12
  • 11
  • 10
  • 7
  • 3
  • 3
  • 2
  • 1
Western Blotting (WB)
  • Binding Specificity

    • 7
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Middle Region

    Sequence

    QDSGQSGSPS HNDPAKNPPG YLEDSFVKSG VFNVSELVRV SRTPITQGTG

    Predicted Reactivity

    Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%, Zebrafish: 77%

    Characteristics

    This is a rabbit polyclonal antibody against NFIB. It was validated on Western Blot using a cell lysate as a positive control.

    Purification

    Protein A purified

    Immunogen

    The immunogen is a synthetic peptide directed towards the middle region of human NFIB
  • Application Notes

    Optimal working dilutions should be determined experimentally by the investigator.

    Comment

    Antigen size: 420 AA

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Avoid repeated freeze-thaw cycles.

    Storage

    -20 °C

    Storage Comment

    For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
  • Lazrek, Goffard, Schanen, Karquel, Bocket, Lion, Devaux, Hedouin, Gosset, Hober: "Detection of hepatitis C virus antibodies and RNA among medicolegal autopsy cases in Northern France." in: Diagnostic microbiology and infectious disease, Vol. 55, Issue 1, pp. 55-8, (2006) (PubMed).

  • Target

    NFIB (Nuclear Factor I/B (NFIB))

    Alternative Name

    NFIB

    Background

    NFIB is a member of the nuclear factor I family of nuclear proteins, which are known to be involved in viral and cellular transcription. NFIB includes the proposed DNA binding and dimerization domain. NFIB recognizes and binds the palindromic sequence 5'-
    Alias Symbols: CTF, NF1-B, NFI-B, NFIB2, NFIB3, NF-I/B, NFI-RED, HMGIC/NFIB
    Protein Interaction Partner: EED, GRK5, SUMO1, ELAVL1, UBC, SOX2, NFIC, RFX1, NFIX,
    Protein Size: 420

    Molecular Weight

    47 kDa

    Gene ID

    4781

    NCBI Accession

    NM_005596, NP_005587

    UniProt

    O00712
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