ACSL1 antibody (C-Term)

Catalog No. ABIN2777590

This Rabbit Polyclonal antibody specifically detects ACSL1 in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Cow, Guinea Pig, Horse, Dog and Zebrafish (Danio rerio).It has been mentioned in 1 publication

Quick Overview for ACSL1 antibody (C-Term) (ABIN2777590)

Target: ACSL1 (Acsl1) (Acyl-CoA Synthetase Long-Chain Family Member 1 (Acsl1))

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Horse, Dog, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ACSL1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-ACSL1 Antibody

Binding Specificity: C-Term

Purpose: ACSL1 Antibody - C-terminal region

Sequence: GSFEELCRNK DVKKAILEDM VRLGKDSGLK PFEQVKGITL HPELFSIDNG

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 79%, Horse: 85%, Human: 100%, Mouse: 100%, Rat: 100%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against ACSL1. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human ACSL1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8093978

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-ACSL1 antibody (ABIN2777590)

Golej, Askari, Kramer, Barnhart, Vivekanandan-Giri, Pennathur, Bornfeldt: "Long-chain acyl-CoA synthetase 4 modulates prostaglandin E? release from human arterial smooth muscle cells." in: Journal of lipid research, Vol. 52, Issue 4, pp. 782-93, (2011) (PubMed).

Target Details for ACSL1

Target: ACSL1 (Acsl1) (Acyl-CoA Synthetase Long-Chain Family Member 1 (Acsl1))

Alternative Name: ACSL1

Background:

Background Information: ACSL1 encodes an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation.The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Gene Name: Acyl-CoA synthetase long-chain family member 1

Alternative Symbols: ACS1, LACS, FACL1, FACL2, LACS1, LACS2

Protein Name: Long-chain-fatty-acid--CoA ligase 1

Molecular Weight: 78kDa

Gene ID: 2180

NCBI Accession: NP_001986

UniProt: P33121

Pathways: Regulation of Lipid Metabolism by PPARalpha