Prohibitin antibody (C-Term)

Catalog No. ABIN2777606

The Rabbit Polyclonal anti-Prohibitin antibody has been validated for WB and IHC. It is suitable to detect Prohibitin in samples from Human, Mouse, Rat, Zebrafish (Danio rerio), Rabbit, Guinea Pig, Cow, Dog and Horse.

Quick Overview for Prohibitin antibody (C-Term) (ABIN2777606)

Target: Prohibitin (PHB)

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Rabbit, Guinea Pig, Cow, Dog, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Prohibitin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Prohibitin Antibody

Binding Specificity: C-Term

Sequence: AEQQKKAAII SAEGDSKAAE LIANSLATAG DGLIELRKLE AAEDIAYQLS

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against PHB. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human PHB

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 272 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for Prohibitin

Target: Prohibitin (PHB)

Alternative Name: PHB

Background: Prohibitin is from an evolutionarily conserved gene that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations in PHB have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region. The longer transcript is present at higher levels in proliferating tissues and cells, suggesting that this longer 3' untranslated region may function as a trans-acting regulatory RNA.
Alias Symbols: PHB1
Protein Interaction Partner: FUS, HUWE1, SUMO2, SPRTN, STAU1, MDM2, SUZ12, EED, RNF2, PARP1, NAP1L1, COX6C, LONRF3, FBXO6, ADRB2, HDAC5, RAF1, KRAS, RAPH1, IPO11, SMG5, YWHAZ, YWHAB, VDAC2, RPS4X, RPL7, PRKCD, PLEC, NPM1, LMNA, IGHG1, HSP90AB1, GTF3C1, DBN1, COL9A2, CAPZA1, SLC25A5,
Protein Size: 272

Molecular Weight: 30 kDa

Gene ID: 5245

NCBI Accession: NM_002634, NP_002625

UniProt: P35232

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling