KDM4C antibody (Middle Region)

Catalog No. ABIN2777688

Cited in 1 publication. The Rabbit Polyclonal anti-KDM4C antibody (ABIN2777688) specifically detects KDM4C in WB. The antibody is reactive with Human, Mouse, Rat, Cow, Dog, Horse, Rabbit, Guinea Pig and Pig samples.

Quick Overview for KDM4C antibody (Middle Region) (ABIN2777688)

Target: KDM4C (Lysine (K)-Specific Demethylase 4C (KDM4C))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Rabbit, Guinea Pig, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KDM4C antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-KDM4C Antibody

Binding Specificity: Middle Region

Purpose: JMJD2C Antibody - C-terminal region

Sequence: CLCNLRGGAL KQTKNNKWAH VMCAVAVPEV RFTNVPERTQ IDVGRIPLQR

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against JMJD2C. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human JMJD2C

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP32974-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-KDM4C antibody (ABIN2777688)

Lu, Ward, Kapoor, Rohle, Turcan, Abdel-Wahab, Edwards, Khanin, Figueroa, Melnick, Wellen, ORourke, Berger, Chan, Levine, Mellinghoff, Thompson: "IDH mutation impairs histone demethylation and results in a block to cell differentiation." in: Nature, Vol. 483, Issue 7390, pp. 474-8, (2012) (PubMed).

Target Details for KDM4C

Target: KDM4C (Lysine (K)-Specific Demethylase 4C (KDM4C))

Alternative Name: JMJD2C

Background:

Background Information: JMJD2C is a member of the Jumonji domain 2 (JMJD2) family. It contains one JmjC domain, one JmjN domain, two PHD-type zinc fingers, and two Tudor domains. This nuclear protein functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated form. Chromosomal aberrations and increased transcriptional expression of this gene are associated with esophageal squamous cell carcinoma.

Gene Name: Lysine (K)-specific demethylase 4C

Alternative Symbols: GASC1, JHDM3C, JMJD2C, TDRD14C

Protein Name: Lysine-specific demethylase 4C Ensembl ENSP00000445427

Molecular Weight: 92 kDa

Gene ID: 23081

NCBI Accession: NP_001140167

UniProt: F5H347

Pathways: Nuclear Hormone Receptor Binding, Warburg Effect