HS3ST1 antibody (Middle Region)

Catalog No. ABIN2777744

This Rabbit Polyclonal antibody specifically detects HS3ST1 in WB. It exhibits reactivity toward Human, Mouse, Rat, Horse, Cow, Dog, Guinea Pig and Rabbit.

Quick Overview for HS3ST1 antibody (Middle Region) (ABIN2777744)

Target: HS3ST1 (Heparan Sulfate (Glucosamine) 3-O-Sulfotransferase 1 (HS3ST1))

Reactivity: Human, Mouse, Rat, Horse, Cow, Dog, Guinea Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HS3ST1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HS3ST1 Antibody

Binding Specificity: Middle Region

Purpose: HS3ST1 Antibody - middle region

Sequence: TKGFYCLRDS GRDRCLHESK GRAHPQVDPK LLNKLHEYFH EPNKKFFELV

Predicted Reactivity: Cow: 92%, Dog: 100%, Guinea Pig: 92%, Horse: 100%, Human: 100%, Mouse: 92%, Rabbit: 92%, Rat: 92%

Characteristics: This is a rabbit polyclonal antibody against HS3ST1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human HS3ST1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP33092-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for HS3ST1

Target: HS3ST1 (Heparan Sulfate (Glucosamine) 3-O-Sulfotransferase 1 (HS3ST1))

Alternative Name: HS3ST1

Background:

Background Information: HS3ST1 is the rate limiting enzyme for synthesis of HSact. HS3ST1 performs the crucial step modification in the biosynthesis of anticoagulant heparan sulfate (HSact) that is to complete the structure of the antithrombin pentasaccharide binding site.Heparan sulfate biosynthetic enzymes are key components in generating a myriad of distinct heparan sulfate fine structures that carry out multiple biologic activities. The enzyme encoded by this gene is a member of the heparan sulfate biosynthetic enzyme family. It possesses both heparan sulfate glucosaminyl 3-O-sulfotransferase activity, anticoagulant heparan sulfate conversion activity, and is a rate limiting enzyme for synthesis of anticoagulant heparan. This enzyme is an intraluminal Golgi resident protein.

Gene Name: Heparan sulfate (glucosamine) 3-O-sulfotransferase 1

Alternative Symbols: 3OST, 3OST1

Protein Name: Heparan sulfate glucosamine 3-O-sulfotransferase 1

Molecular Weight: 36 kDa

Gene ID: 9957

NCBI Accession: NP_005105

UniProt: O14792

Pathways: Glycosaminoglycan Metabolic Process