MAP4K5 antibody (Middle Region)

Catalog No. ABIN2777750

Product Details anti-MAP4K5 Antibody

Target: MAP4K5 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 5 (MAP4K5))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio), Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP4K5 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: QGKSFKSDEV TQEISDETRV FRLLGSDRVV VLESRPTENP TAHSNLYILA

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 77%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against MAP4K5. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human MAP4K5

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 846 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for MAP4K5

Target: MAP4K5 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 5 (MAP4K5))

Alternative Name: MAP4K5 (MAP4K5 Products)

Background: MAP4K5 may play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway.This gene encodes a member of the serine/threonine protein kinase family, that is highly similar to yeast SPS1/STE20 kinase. Yeast SPS1/STE20 functions near the beginning of the MAP kinase signal cascades that is essential for yeast pheromone response. This kinase was shown to activate Jun kinase in mammalian cells, which suggested a role in stress response. Two alternatively spliced transcript variants encoding the same protein have been described for this gene.
Alias Symbols: GCKR, KHS, KHS1, MAPKKKK5
Protein Interaction Partner: CRK, ELAVL1, UBC, NEDD4, SETDB1, ZNF440, COPS5, HCK, GRB2, TANK, CRKL, TRAF2, ABL1, BCR,
Protein Size: 846

Molecular Weight: 95 kDa

Gene ID: 11183

NCBI Accession: NM_006575, NP_006566

UniProt: Q9Y4K4