Diazepam Binding Inhibitor antibody (N-Term)

Catalog No. ABIN2777771

This Rabbit Polyclonal antibody specifically detects Diazepam Binding Inhibitor in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Guinea Pig and Rabbit.

Quick Overview for Diazepam Binding Inhibitor antibody (N-Term) (ABIN2777771)

Target: Diazepam Binding Inhibitor (DBI)

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Diazepam Binding Inhibitor antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Diazepam Binding Inhibitor Antibody

Binding Specificity: N-Term

Sequence: MSQAEFEKAA EEVRHLKTKP SDEEMLFIYG HYKQATVGDI NTERPGMLDF

Predicted Reactivity: Cow: 86%, Dog: 79%, Guinea Pig: 92%, Human: 100%, Mouse: 85%, Rabbit: 86%, Rat: 92%

Characteristics: This is a rabbit polyclonal antibody against DBI. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human DBI

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 87 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for Diazepam Binding Inhibitor

Target: Diazepam Binding Inhibitor (DBI)

Alternative Name: DBI

Background: DBI is diazepam binding inhibitor. The protein that is regulated by hormones and is involved in lipid metabolism and the displacement of beta-carbolines and benzodiazepines, which modulate signal transduction at type A gamma-aminobutyric acid receptors located in brain synapses. DBI is conserved from yeast to mammals, with the most highly conserved domain consisting of seven contiguous residues that constitute the hydrophobic binding site for medium- and long-chain acyl-Coenzyme A esters. Diazepam binding inhibitor is also known to mediate the feedback regulation of pancreatic secretion and the postprandial release of cholecystokinin, in addition to its role as a mediator in corticotropin-dependent adrenal steroidogenesis. Three pseudogenes located on chromosomes 6, 8 and 16 have been identified. Multiple transcript variants encoding different isoforms have been described for this gene. This gene encodes diazepam binding inhibitor, a protein that is regulated by hormones and is involved in lipid metabolism and the displacement of beta-carbolines and benzodiazepines, which modulate signal transduction at type A gamma-aminobutyric acid receptors located in brain synapses. The protein is conserved from yeast to mammals, with the most highly conserved domain consisting of seven contiguous residues that constitute the hydrophobic binding site for medium- and long-chain acyl-Coenzyme A esters. Diazepam binding inhibitor is also known to mediate the feedback regulation of pancreatic secretion and the postprandial release of cholecystokinin, in addition to its role as a mediator in corticotropin-dependent adrenal steroidogenesis. Three pseudogenes located on chromosomes 6, 8 and 16 have been identified. Multiple transcript variants encoding different isoforms have been described for this gene.
Alias Symbols: ACBD1, ACBP, CCK-RP, EP, MGC70414
Protein Interaction Partner: UBC, MAP1LC3B, NSFL1C, DUSP23, NIPSNAP1, RAD23B, RAD23A, GSK3B, UBXN2A, TSPO, PPCDC,
Protein Size: 87

Molecular Weight: 10 kDa

Gene ID: 1622

NCBI Accession: NM_001079862, NP_001073331

UniProt: P07108