SOX5 antibody (C-Term)

Catalog No. ABIN2777862

Product Details anti-SOX5 Antibody

Target: SOX5 (SRY (Sex Determining Region Y)-Box 5 (SOX5))

Binding Specificity: C-Term

Reactivity: Human, Rat, Mouse, Guinea Pig, Horse, Rabbit, Cow, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SOX5 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: PEPGMPVIQS TYGVKGEEPH IKEEIQAEDI NGEIYDEYDE EEDDPDVDYG

Specificity: Designed to recognize all five isoforms of human SOX5 (84, 82, 42, 71 and 83 kDa)

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 92%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against SOX5. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human SOX5

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 377 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-SOX5 antibody (ABIN2777862)

Zhang, Liu, Li, Zhang, Li, Liu, Min, Shuang, Song, Zhang: "Transcriptional regulation of human sperm-associated antigen 16 gene by S-SOX5." in: BMC molecular biology, Vol. 18, Issue 1, pp. 2, (2017) (PubMed).

Naveau, Pinson, Gérard, Nguyen, Charlier, Thomé, Zoeller, Bourguignon, Parent: "Alteration of rat fetal cerebral cortex development after prenatal exposure to polychlorinated biphenyls." in: PLoS ONE, Vol. 9, Issue 3, pp. e91903, (2014) (PubMed).

Fernandes, Herlofsen, Karlsen, Küchler, Fløisand, Brinchmann: "Similar properties of chondrocytes from osteoarthritis joints and mesenchymal stem cells from healthy donors for tissue engineering of articular cartilage." in: PLoS ONE, Vol. 8, Issue 5, pp. e62994, (2013) (PubMed).

Boije, Harun-Or-Rashid, Lee, Imsland, Bruneau, Vieaud, Gourichon, Tixier-Boichard, Bedhom, Andersson, Hallböök: "Sonic Hedgehog-signalling patterns the developing chicken comb as revealed by exploration of the pea-comb mutation." in: PLoS ONE, Vol. 7, Issue 12, pp. e50890, (2012) (PubMed).

Yu, Deshmukh, Payton, Dunham, Scheithauer, Tihan, Prayson, Guha, Bridge, Ferner, Lindberg, Gutmann, Emnett, Salavaggione, Gutmann, Nagarajan, Watson, Perry: "Array-based comparative genomic hybridization identifies CDK4 and FOXM1 alterations as independent predictors of survival in malignant peripheral nerve sheath tumor." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 17, Issue 7, pp. 1924-34, (2011) (PubMed).

Aza-Carmona, Shears, Yuste-Checa, Barca-Tierno, Hisado-Oliva, Belinchón, Benito-Sanz, Rodríguez, Argente, Campos-Barros, Scambler, Heath: "SHOX interacts with the chondrogenic transcription factors SOX5 and SOX6 to activate the aggrecan enhancer." in: Human molecular genetics, Vol. 20, Issue 8, pp. 1547-59, (2011) (PubMed).

Kiselak, Shen, Song, Gude, Wang, Brody, Strauss, Zhang: "Transcriptional regulation of an axonemal central apparatus gene, sperm-associated antigen 6, by a SRY-related high mobility group transcription factor, S-SOX5." in: The Journal of biological chemistry, Vol. 285, Issue 40, pp. 30496-505, (2010) (PubMed).

Pytel, Karrison, Can Gong, Tonsgard, Krausz, Montag: "Neoplasms with schwannian differentiation express transcription factors known to regulate normal schwann cell development." in: International journal of surgical pathology, Vol. 18, Issue 6, pp. 449-57, (2010) (PubMed).

Torii, Hashimoto-Torii, Levitt, Rakic: "Integration of neuronal clones in the radial cortical columns by EphA and ephrin-A signalling." in: Nature, Vol. 461, Issue 7263, pp. 524-8, (2009) (PubMed).

Wright, Boije, Meadows, Bedhom, Gourichon, Vieaud, Tixier-Boichard, Rubin, Imsland, Hallböök, Andersson: "Copy number variation in intron 1 of SOX5 causes the Pea-comb phenotype in chickens." in: PLoS genetics, Vol. 5, Issue 6, pp. e1000512, (2009) (PubMed).

Tchougounova, Jiang, Bråsäter, Lindberg, Kastemar, Asplund, Westermark, Uhrbom: "Sox5 can suppress platelet-derived growth factor B-induced glioma development in Ink4a-deficient mice through induction of acute cellular senescence." in: Oncogene, Vol. 28, Issue 12, pp. 1537-48, (2009) (PubMed).

Martelli, Isella, Mira, Fu, Cantarella, Medico: "Exploiting orthologue diversity for systematic detection of gain-of-function phenotypes." in: BMC genomics, Vol. 9, pp. 254, (2008) (PubMed).

Target Details for SOX5

Target: SOX5 (SRY (Sex Determining Region Y)-Box 5 (SOX5))

Alternative Name: SOX5 (SOX5 Products)

Synonyms: L-SOX5 antibody, XSox-5 antibody, l-sox5 antibody, A730017D01Rik antibody, AI528773 antibody, Sox5l1 antibody, xSox5 antibody, id:ibd5067 antibody, sb:eu456 antibody, si:dkey-215a8.1 antibody, SRY-box 5 antibody, SRY (sex determining region Y)-box 5 antibody, SRY box 5 antibody, SRY-box 5 S homeolog antibody, SOX5 antibody, sox5 antibody, Sox5 antibody, sox5.S antibody

Background: SOX5 encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins. The encoded protein may play a role in chondrogenesis. A pseudogene of this gene is located on chromosome 8.
Alias Symbols: L-SOX5, MGC35153
Protein Interaction Partner: SUMO1P1, MORN3, PRR20A, FAM46B, CBX8, ZNF581, ARID5A, KAT5, CDC23, TAF6, SOX5, LMO2, LMO1, KIFC3, CRX, AES, MED27, UQCRFS1, TTC1, RPS2, SMAD7, SMAD5, SMAD1, FTH1, CDK6, CDC25A, APP, SOX2, SOX6,
Protein Size: 377

Molecular Weight: 42 kDa

Gene ID: 6660

NCBI Accession: NM_178010, NP_821078

UniProt: P35711