ING3 antibody (Middle Region)

Catalog No. ABIN2777878

The Rabbit Polyclonal anti-ING3 antibody is suitable to detect ING3 in samples from Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio) and Saccharomyces cerevisiae. It has been validated for WB and IHC.

Quick Overview for ING3 antibody (Middle Region) (ABIN2777878)

Target: ING3 (Inhibitor of Growth Family, Member 3 (ING3))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio), Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ING3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-ING3 Antibody

Binding Specificity: Middle Region

Purpose: ING3 Antibody - middle region

Sequence: LSSGTGAGAI TMAAAQAVQA TAQMKEGRRT SSLKASYEAF KNNDFQLGKE

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 75%, Zebrafish: 92%

Characteristics: This is a rabbit polyclonal antibody against ING3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ING3

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8103049

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for ING3

Target: ING3 (Inhibitor of Growth Family, Member 3 (ING3))

Alternative Name: ING3

Background:

Background Information: ING3 is similar to ING1, a tumor suppressor protein that can interact with TP53, inhibit cell growth, and induce apoptosis. This protein contains a PHD-finger, which is a common motif in proteins involved in chromatin remodeling. This gene can activate p53 trans-activated promoters, including promoters of p21/waf1 and bax. Overexpression of this gene has been shown to inhibit cell growth and induce apoptosis. Allelic loss and reduced expression of this gene were detected in head and neck cancers.The protein encoded by this gene is similar to ING1, a tumor suppressor protein that can interact with TP53, inhibit cell growth, and induce apoptosis. This protein contains a PHD-finger, which is a common motif in proteins involved in chromatin remodeling. This gene can activate p53 trans-activated promoters, including promoters of p21/waf1 and bax. Overexpression of this gene has been shown to inhibit cell growth and induce apoptosis. Allelic loss and reduced expression of this gene were detected in head and neck cancers. Two alternatively spliced transcript variants encoding different isoforms have been observed.

Gene Name: Inhibitor of growth family, member 3

Alternative Symbols: Eaf4, ING2, MEAF4, p47ING3

Protein Name: Inhibitor of growth protein 3

Molecular Weight: 47kDa

Gene ID: 54556

NCBI Accession: NP_061944

UniProt: Q9NXR8