TEAD4 antibody (Middle Region)

Catalog No. ABIN2777935

Cited in 1 publication. The Rabbit Polyclonal anti-TEAD4 antibody (ABIN2777935) specifically detects TEAD4 in WB and IF. The antibody is reactive with Human, Mouse, Rat, Guinea Pig, Horse, Rabbit, Cow and Dog samples.

Quick Overview for TEAD4 antibody (Middle Region) (ABIN2777935)

Target: TEAD4 (TEA Domain Family Member 4 (TEAD4))

Reactivity: Human, Mouse, Rat, Guinea Pig, Horse, Rabbit, Cow, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TEAD4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-TEAD4 Antibody

Binding Specificity: Middle Region

Purpose: TEAD4 Antibody - middle region

Sequence: LVKFWADLNT NIEDEGSSFY GVSSQYESPE NMIITCSTKV CSFGKQVVEK

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 85%, Rabbit: 100%, Rat: 77%

Characteristics: This is a rabbit polyclonal antibody against TEAD4. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human TEAD4

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8113596

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-TEAD4 antibody (ABIN2777935)

Chan, Lim, Loo, Chong, Huang, Hong: "TEADs mediate nuclear retention of TAZ to promote oncogenic transformation." in: The Journal of biological chemistry, Vol. 284, Issue 21, pp. 14347-58, (2009) (PubMed).

Target Details for TEAD4

Target: TEAD4 (TEA Domain Family Member 4 (TEAD4))

Alternative Name: TEAD4

Background:

Background Information: TEAD4 is a member of the transcriptional enhancer factor (TEF) family of transcription factors, which contain the TEA/ATTS DNA-binding domain. TEAD4 is preferentially expressed in the skeletal muscle, and binds to the M-CAT regulatory element found in promoters of muscle-specific genes to direct their gene expression. Alternatively spliced transcripts encoding distinct isoforms, some of which are translated through the use of a non-AUG (UUG) initiation codon, have been described for this protein.This gene product is a member of the transcriptional enhancer factor (TEF) family of transcription factors, which contain the TEA/ATTS DNA-binding domain. It is preferentially expressed in the skeletal muscle, and binds to the M-CAT regulatory element found in promoters of muscle-specific genes to direct their gene expression. Alternatively spliced transcripts encoding distinct isoforms, some of which are translated through the use of a non-AUG (UUG) initiation codon, have been described for this gene.

Gene Name: TEA domain family member 4

Alternative Symbols: TEF3, RTEF1, TEF-3, EFTR-2, TEFR-1, TCF13L1, hRTEF-1B

Protein Name: Transcriptional enhancer factor TEF-3

Molecular Weight: 44kDa

Gene ID: 7004

NCBI Accession: NP_958849

UniProt: Q15561

Pathways: Regulation of Lipid Metabolism by PPARalpha